1mk0
From Proteopedia
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|PDB= 1mk0 |SIZE=350|CAPTION= <scene name='initialview01'>1mk0</scene>, resolution 1.60Å | |PDB= 1mk0 |SIZE=350|CAPTION= <scene name='initialview01'>1mk0</scene>, resolution 1.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ln0|1LN0]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mk0 OCA], [http://www.ebi.ac.uk/pdbsum/1mk0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mk0 RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1MK0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1MK0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MK0 OCA]. |
==Reference== | ==Reference== | ||
Catalytic domain structure and hypothesis for function of GIY-YIG intron endonuclease I-TevI., Van Roey P, Meehan L, Kowalski JC, Belfort M, Derbyshire V, Nat Struct Biol. 2002 Nov;9(11):806-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12379841 12379841] | Catalytic domain structure and hypothesis for function of GIY-YIG intron endonuclease I-TevI., Van Roey P, Meehan L, Kowalski JC, Belfort M, Derbyshire V, Nat Struct Biol. 2002 Nov;9(11):806-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12379841 12379841] | ||
| - | [[Category: | + | [[Category: Enterobacteria phage t4]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Belfort, M.]] | [[Category: Belfort, M.]] | ||
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[[Category: Meehan, L.]] | [[Category: Meehan, L.]] | ||
[[Category: Roey, P Van.]] | [[Category: Roey, P Van.]] | ||
| - | [[Category: | + | [[Category: alpha/beta fold]] |
| - | [[Category: | + | [[Category: catalytic domain]] |
| - | [[Category: | + | [[Category: dna-binding surface]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:17:02 2008'' |
Revision as of 19:17, 30 March 2008
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| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1LN0
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
catalytic domain of intron endonuclease I-TevI, E75A mutant
Overview
I-TevI, a member of the GIY-YIG family of homing endonucleases, consists of an N-terminal catalytic domain and a C-terminal DNA-binding domain joined by a flexible linker. The GIY-YIG motif is in the N-terminal domain of I-TevI, which corresponds to a phylogenetically widespread catalytic cartridge that is often associated with mobile genetic elements. The crystal structure of the catalytic domain of I-TevI, the first of any GIY-YIG endonuclease, reveals a novel alpha/beta-fold with a central three-stranded antiparallel beta-sheet flanked by three helices. The most conserved and putative catalytic residues are located on a shallow, concave surface and include a metal coordination site. Similarities in the three-dimensional arrangement of the catalytically important residues and the cation-binding site with those of the His-Cys box endonuclease I-PpoI suggest the possibility of mechanistic relationships among these different families of homing endonucleases despite completely different folds.
About this Structure
1MK0 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Catalytic domain structure and hypothesis for function of GIY-YIG intron endonuclease I-TevI., Van Roey P, Meehan L, Kowalski JC, Belfort M, Derbyshire V, Nat Struct Biol. 2002 Nov;9(11):806-11. PMID:12379841
Page seeded by OCA on Sun Mar 30 22:17:02 2008
