4kr1

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(Difference between revisions)

Revision as of 15:04, 5 August 2016

Crystal structure of the kinetechore protein Iml3 from budding yeast

Structural highlights

4kr1 is a 1 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	IML3, MCM19, YBR107C, YBR0836 (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IML3_YEAST] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.^[1] ^[2]

Publication Abstract from PubMed

Human CENP-N and CENP-L have been reported to selectively recognize the CENP-A nucleosome and to contribute to recruiting other constitutive centromere-associated network (CCAN) complexes involved in assembly of the inner kinetochore. As their homologues, Chl4 and Iml3 from budding yeast function in a similar way in de novo assembly of the kinetochore. A lack of biochemical and structural information precludes further understanding of their exact role at the molecular level. Here, the crystal structure of Iml3 is presented and the structure shows that Iml3 adopts an elongated conformation with a series of intramolecular interactions. Pull-down assays revealed that the C-terminal domain of Chl4, which forms a dimer in solution, is responsible for Iml3 binding. Acting as a heterodimer, the Chl4-Iml3 complex exhibits a low-affinity nonspecific DNA-binding activity which may play an important role in the kinetochore-assembly process.

Structural insights into the role of the Chl4-Iml3 complex in kinetochore assembly.,Guo Q, Tao Y, Liu H, Teng M, Li X Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2412-9. doi:, 10.1107/S0907444913022397. Epub 2013 Nov 19. PMID:24311582^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ghosh SK, Poddar A, Hajra S, Sanyal K, Sinha P. The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a kinetochore-related process during chromosome segregation. Mol Genet Genomics. 2001 Apr;265(2):249-57. PMID:11361335
↑ Ghosh SK, Sau S, Lahiri S, Lohia A, Sinha P. The Iml3 protein of the budding yeast is required for the prevention of precocious sister chromatid separation in meiosis I and for sister chromatid disjunction in meiosis II. Curr Genet. 2004 Aug;46(2):82-91. Epub 2004 Jul 6. PMID:15241623 doi:http://dx.doi.org/10.1007/s00294-004-0516-6
↑ Guo Q, Tao Y, Liu H, Teng M, Li X. Structural insights into the role of the Chl4-Iml3 complex in kinetochore assembly. Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2412-9. doi:, 10.1107/S0907444913022397. Epub 2013 Nov 19. PMID:24311582 doi:http://dx.doi.org/10.1107/S0907444913022397

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4kr1"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the kinetechore protein Iml3 from budding yeast==
 <StructureSection load='4kr1' size='340' side='right' caption='[[4kr1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IML3, MCM19, YBR107C, YBR0836 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kr1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kr1 RCSB], [http://www.ebi.ac.uk/pdbsum/4kr1 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kr1 OCA], [http://pdbe.org/4kr1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kr1 RCSB], [http://www.ebi.ac.uk/pdbsum/4kr1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kr1 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4kr1" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4kr1

From Proteopedia

Revision as of 15:04, 5 August 2016

Crystal structure of the kinetechore protein Iml3 from budding yeast

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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