4if4
From Proteopedia
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| - | {{STRUCTURE_4if4| PDB=4if4 | SCENE= }} | ||
| - | ===Crystal Structure of the Magnesium and beryllofluoride-activated VraR from Staphylococcus aureus=== | ||
| - | {{ABSTRACT_PUBMED_23650349}} | ||
| - | == | + | ==Crystal Structure of the Magnesium and beryllofluoride-activated VraR from Staphylococcus aureus== |
| - | [[4if4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | <StructureSection load='4if4' size='340' side='right' caption='[[4if4]], [[Resolution|resolution]] 2.35Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4if4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IF4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gvp|4gvp]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAV1884, vraR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4if4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4if4 OCA], [http://pdbe.org/4if4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4if4 RCSB], [http://www.ebi.ac.uk/pdbsum/4if4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4if4 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/VRAR_STAAM VRAR_STAAM]] Member of the two-component regulatory system VraS/VraR involved in the control of the cell wall peptidoglycan biosynthesis. VraR is overexpressed in strain Mu50, which leads to vancomycin resistance. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Staphylococcus aureus VraR, a vancomycin-resistance-associated response regulator, activates a cell-wall-stress stimulon in response to antibiotics that inhibit cell wall formation. X-ray crystal structures of VraR in both unphosphorylated and beryllofluoride-activated states have been determined, revealing a mechanism of phosphorylation-induced dimerization that features a deep hydrophobic pocket at the center of the receiver domain interface. Unphosphorylated VraR exists in a closed conformation that inhibits dimer formation. Phosphorylation at the active site promotes conformational changes that are propagated throughout the receiver domain, promoting the opening of a hydrophobic pocket that is essential for homodimer formation and enhanced DNA-binding activity. This prominent feature in the VraR dimer can potentially be exploited for the development of novel therapeutics to counteract antibiotic resistance in this important pathogen. | ||
| - | + | Phosphorylation-dependent conformational changes and domain rearrangements in Staphylococcus aureus VraR activation.,Leonard PG, Golemi-Kotra D, Stock AM Proc Natl Acad Sci U S A. 2013 May 21;110(21):8525-30. doi:, 10.1073/pnas.1302819110. Epub 2013 May 6. PMID:23650349<ref>PMID:23650349</ref> | |
| - | <ref | + | |
| - | [[Category: | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | [[Category: Leonard, P G | + | </div> |
| - | [[Category: Stock, A M | + | <div class="pdbe-citations 4if4" style="background-color:#fffaf0;"></div> |
| + | |||
| + | ==See Also== | ||
| + | *[[Response regulator|Response regulator]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Staam]] | ||
| + | [[Category: Leonard, P G]] | ||
| + | [[Category: Stock, A M]] | ||
[[Category: Bacterial signalling]] | [[Category: Bacterial signalling]] | ||
[[Category: Dna binding]] | [[Category: Dna binding]] | ||
Revision as of 16:20, 5 August 2016
Crystal Structure of the Magnesium and beryllofluoride-activated VraR from Staphylococcus aureus
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