1mr1
From Proteopedia
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|PDB= 1mr1 |SIZE=350|CAPTION= <scene name='initialview01'>1mr1</scene>, resolution 2.85Å | |PDB= 1mr1 |SIZE=350|CAPTION= <scene name='initialview01'>1mr1</scene>, resolution 2.85Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ygs|1YGS]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mr1 OCA], [http://www.ebi.ac.uk/pdbsum/1mr1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mr1 RCSB]</span> | ||
}} | }} | ||
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==Overview== | ==Overview== | ||
The Ski family of nuclear oncoproteins represses TGF-beta signaling through interactions with the Smad proteins. The crystal structure of the Smad4 binding domain of human c-Ski in complex with the MH2 domain of Smad4 reveals specific recognition of the Smad4 L3 loop region by a highly conserved interaction loop (I loop) from Ski. The Ski binding surface on Smad4 significantly overlaps with that required for binding of the R-Smads. Indeed, Ski disrupts the formation of a functional complex between the Co- and R-Smads, explaining how it could lead to repression of TGF-beta, activin, and BMP responses. Intriguingly, the structure of the Ski fragment, stabilized by a bound zinc atom, resembles the SAND domain, in which the corresponding I loop is responsible for DNA binding. | The Ski family of nuclear oncoproteins represses TGF-beta signaling through interactions with the Smad proteins. The crystal structure of the Smad4 binding domain of human c-Ski in complex with the MH2 domain of Smad4 reveals specific recognition of the Smad4 L3 loop region by a highly conserved interaction loop (I loop) from Ski. The Ski binding surface on Smad4 significantly overlaps with that required for binding of the R-Smads. Indeed, Ski disrupts the formation of a functional complex between the Co- and R-Smads, explaining how it could lead to repression of TGF-beta, activin, and BMP responses. Intriguingly, the structure of the Ski fragment, stabilized by a bound zinc atom, resembles the SAND domain, in which the corresponding I loop is responsible for DNA binding. | ||
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| - | ==Disease== | ||
| - | Known diseases associated with this structure: 1p36 deletion syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=164780 164780]], Juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600993 600993]], Pancreatic cancer OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600993 600993]], Polyposis, juvenile intestinal OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600993 600993]] | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Wu, J W.]] | [[Category: Wu, J W.]] | ||
[[Category: Zhang, F.]] | [[Category: Zhang, F.]] | ||
| - | [[Category: ZN]] | ||
[[Category: cancer]] | [[Category: cancer]] | ||
[[Category: protein interaction]] | [[Category: protein interaction]] | ||
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[[Category: tgf-b signaling]] | [[Category: tgf-b signaling]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:19:55 2008'' |
Revision as of 19:19, 30 March 2008
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| , resolution 2.85Å | |||||||
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| Ligands: | |||||||
| Related: | 1YGS
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of a Smad4-Ski Complex
Overview
The Ski family of nuclear oncoproteins represses TGF-beta signaling through interactions with the Smad proteins. The crystal structure of the Smad4 binding domain of human c-Ski in complex with the MH2 domain of Smad4 reveals specific recognition of the Smad4 L3 loop region by a highly conserved interaction loop (I loop) from Ski. The Ski binding surface on Smad4 significantly overlaps with that required for binding of the R-Smads. Indeed, Ski disrupts the formation of a functional complex between the Co- and R-Smads, explaining how it could lead to repression of TGF-beta, activin, and BMP responses. Intriguingly, the structure of the Ski fragment, stabilized by a bound zinc atom, resembles the SAND domain, in which the corresponding I loop is responsible for DNA binding.
About this Structure
1MR1 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural mechanism of Smad4 recognition by the nuclear oncoprotein Ski: insights on Ski-mediated repression of TGF-beta signaling., Wu JW, Krawitz AR, Chai J, Li W, Zhang F, Luo K, Shi Y, Cell. 2002 Nov 1;111(3):357-67. PMID:12419246
Page seeded by OCA on Sun Mar 30 22:19:55 2008
Categories: Homo sapiens | Protein complex | Chai, J. | Krawitz, A R. | Li, W. | Luo, K. | Shi, Y. | Wu, J W. | Zhang, F. | Cancer | Protein interaction | Ski | Smad | Tgf-b signaling
