1mu4

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|PDB= 1mu4 |SIZE=350|CAPTION= <scene name='initialview01'>1mu4</scene>, resolution 1.80&Aring;
|PDB= 1mu4 |SIZE=350|CAPTION= <scene name='initialview01'>1mu4</scene>, resolution 1.80&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=[[1mo1|1MO1]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mu4 OCA], [http://www.ebi.ac.uk/pdbsum/1mu4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mu4 RCSB]</span>
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}}
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[[Category: Haser, R.]]
[[Category: Haser, R.]]
[[Category: Juy, M R.]]
[[Category: Juy, M R.]]
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[[Category: SO4]]
 
[[Category: open-faced b-sandwich]]
[[Category: open-faced b-sandwich]]
[[Category: phosphotransferase system]]
[[Category: phosphotransferase system]]
[[Category: swapping domain]]
[[Category: swapping domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:48:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:21:07 2008''

Revision as of 19:21, 30 March 2008


PDB ID 1mu4

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands:
Related: 1MO1


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE AT 1.8 ANGSTROMS OF THE BACILLUS SUBTILIS CATABOLITE REPRESSION HISTIDINE CONTAINING PROTEIN (CRH)


Overview

The crystal structure of the regulatory protein Crh from Bacillus subtilis was solved at 1.8A resolution and showed an intertwined dimer formed by N-terminal beta1-strand swapping of two monomers. Comparison with the monomeric NMR structure of Crh revealed a domain swap induced conformational rearrangement of the putative interaction site with the repressor CcpA. The resulting conformation closely resembles that observed for the monomeric Crh homologue HPr, indicating that the Crh dimer is the active form binding to CcpA. An analogous dimer of HPr can be constructed without domain swapping, suggesting that HPr may dimerize upon binding to CcpA. Our data suggest that reversible 3D domain swapping of Crh might be an efficient regulatory mechanism to modulate its activity.

About this Structure

1MU4 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Dimerization of Crh by reversible 3D domain swapping induces structural adjustments to its monomeric homologue Hpr., Juy M, Penin F, Favier A, Galinier A, Montserret R, Haser R, Deutscher J, Bockmann A, J Mol Biol. 2003 Sep 26;332(4):767-76. PMID:12972249

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