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4a2a

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Revision as of 19:51, 5 August 2016

Thermotoga maritima FtsA:FtsZ(336-351)

Structural highlights

4a2a is a 4 chain structure with sequence from Atcc 43589. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1w5f, 1e4g, 1e4f, 4a2b
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FTSZ_THEMA] Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.^[1]

Publication Abstract from PubMed

FtsA is an early component of the Z-ring, the structure that divides most bacteria, formed by tubulin-like FtsZ. FtsA belongs to the actin family of proteins, showing an unusual subdomain architecture. Here we reconstitute the tethering of FtsZ to the membrane via FtsA's C-terminal amphipathic helix in vitro using Thermotoga maritima proteins. A crystal structure of the FtsA:FtsZ interaction reveals 16 amino acids of the FtsZ tail bound to subdomain 2B of FtsA. The same structure and a second crystal form of FtsA reveal that FtsA forms actin-like protofilaments with a repeat of 48 A. The identical repeat is observed when FtsA is polymerized using a lipid monolayer surface and FtsAs from three organisms form polymers in cells when overexpressed, as observed by electron cryotomography. Mutants that disrupt polymerization also show an elongated cell division phenotype in a temperature-sensitive FtsA background, demonstrating the importance of filament formation for FtsA's function in the Z-ring.

FtsA forms actin-like protofilaments.,Szwedziak P, Wang Q, Freund SM, Lowe J EMBO J. 2012 Mar 30. doi: 10.1038/emboj.2012.76. PMID:22473211^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lu C, Stricker J, Erickson HP. FtsZ from Escherichia coli, Azotobacter vinelandii, and Thermotoga maritima--quantitation, GTP hydrolysis, and assembly. Cell Motil Cytoskeleton. 1998;40(1):71-86. PMID:9605973 doi:<71::AID-CM7>3.0.CO;2-I http://dx.doi.org/10.1002/(SICI)1097-0169(1998)40:1<71::AID-CM7>3.0.CO;2-I
↑ Szwedziak P, Wang Q, Freund SM, Lowe J. FtsA forms actin-like protofilaments. EMBO J. 2012 Mar 30. doi: 10.1038/emboj.2012.76. PMID:22473211 doi:10.1038/emboj.2012.76

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4a2a"

@@ Line 1: / Line 1: @@
 ==Thermotoga maritima FtsA:FtsZ(336-351)==
 <StructureSection load='4a2a' size='340' side='right' caption='[[4a2a]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4a2a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A2A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4a2a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A2A FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1w5f|1w5f]], [[1e4g|1e4g]], [[1e4f|1e4f]], [[4a2b|4a2b]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a2a RCSB], [http://www.ebi.ac.uk/pdbsum/4a2a PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a2a OCA], [http://pdbe.org/4a2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a2a RCSB], [http://www.ebi.ac.uk/pdbsum/4a2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a2a ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4a2a" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Thermotoga maritima]]
+[[Category: Atcc 43589]]
 [[Category: Lowe, J]]
 [[Category: Szwedziak, P]]

4a2a

From Proteopedia

Revision as of 19:51, 5 August 2016

Thermotoga maritima FtsA:FtsZ(336-351)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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