1n1p
From Proteopedia
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|PDB= 1n1p |SIZE=350|CAPTION= <scene name='initialview01'>1n1p</scene>, resolution 0.95Å | |PDB= 1n1p |SIZE=350|CAPTION= <scene name='initialview01'>1n1p</scene>, resolution 0.95Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] </span> |
|GENE= CHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Streptomyces sp.]) | |GENE= CHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Streptomyces sp.]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1b4v|1B4V]], [[1mxt|1MXT]], [[1b8s|1B8S]], [[1cc2|1CC2]], [[1ijh|1IJH]], [[1cbo|1CBO]], [[1n4v|1N4V]], [[1n4w|1N4W]], [[1n4u|1N4U]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n1p OCA], [http://www.ebi.ac.uk/pdbsum/1n1p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n1p RCSB]</span> | ||
}} | }} | ||
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[[Category: Lario, P I.]] | [[Category: Lario, P I.]] | ||
[[Category: Vrielink, A.]] | [[Category: Vrielink, A.]] | ||
| - | [[Category: FAD]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: MN]] | ||
[[Category: atomic resolution]] | [[Category: atomic resolution]] | ||
[[Category: flavoenzyme]] | [[Category: flavoenzyme]] | ||
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[[Category: steroid metabolism]] | [[Category: steroid metabolism]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:24:07 2008'' |
Revision as of 19:24, 30 March 2008
| |||||||
| , resolution 0.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | CHOA (Streptomyces sp.) | ||||||
| Activity: | Cholesterol oxidase, with EC number 1.1.3.6 | ||||||
| Related: | 1B4V, 1MXT, 1B8S, 1CC2, 1IJH, 1CBO, 1N4V, 1N4W, 1N4U
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ pH 7.4 (STREPTOMYCES SP. SA-COO)
Overview
The X-ray crystal structure of the flavoenzyme cholesterol oxidase, SCOA (Streptomyces sp.SA-COO) has been determined to 0.95 A resolution. The large size (55kDa) and the high-resolution diffraction of this protein provides a unique opportunity to observe detailed electronic effects within a protein environment and to obtain a larger sampling for which to analyze these electronic and structural differences. It is well-known through spectroscopic methods that peptide carbonyl groups are polarized in alpha-helices. This electronic characteristic is evident in the sub-Angstrom electron density of SCOA. Our analysis indicates an increased tendency for the electron density of the main chain carbonyl groups within alpha-helices to be polarized toward the oxygen atoms. In contrast, the carbonyl groups in beta-sheet structures tend to exhibit a greater charge density between the carbon and oxygen atoms. Interestingly, the electronic differences observed at the carbonyl groups do not appear to be correlated to the bond distance of the peptide bond or the peptide planarity. This study gives important insight into the electronic effects of alpha-helix dipoles in enzymes and provides experimentally based observations that have not been previously characterized in protein structure.
About this Structure
1N1P is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.
Reference
Atomic resolution density maps reveal secondary structure dependent differences in electronic distribution., Lario PI, Vrielink A, J Am Chem Soc. 2003 Oct 22;125(42):12787-94. PMID:14558826
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