5l7j
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l7j OCA], [http://pdbe.org/5l7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l7j RCSB], [http://www.ebi.ac.uk/pdbsum/5l7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l7j ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l7j OCA], [http://pdbe.org/5l7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l7j RCSB], [http://www.ebi.ac.uk/pdbsum/5l7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l7j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | During translation elongation, decoding is based on the recognition of codons by corresponding tRNA anticodon triplets. Molecular mechanisms that regulate global protein synthesis via specific base modifications in tRNA anticodons are receiving increasing attention. The conserved eukaryotic Elongator complex specifically modifies uridines located in the wobble base position of tRNAs. Mutations in Elongator subunits are associated with certain neurodegenerative diseases and cancer. Here we present the crystal structure of D. mccartyi Elp3 (DmcElp3) at 2.15-A resolution. Our results reveal an unexpected arrangement of Elp3 lysine acetyltransferase (KAT) and radical S-adenosyl methionine (SAM) domains, which share a large interface and form a composite active site and tRNA-binding pocket, with an iron-sulfur cluster located in the dimerization interface of two DmcElp3 molecules. Structure-guided mutagenesis studies of yeast Elp3 confirmed the relevance of our findings for eukaryotic Elp3s and should aid in understanding the cellular functions and pathophysiological roles of Elongator. | ||
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| + | Structural basis for tRNA modification by Elp3 from Dehalococcoides mccartyi.,Glatt S, Zabel R, Kolaj-Robin O, Onuma OF, Baudin F, Graziadei A, Taverniti V, Lin TY, Baymann F, Seraphin B, Breunig KD, Muller CW Nat Struct Mol Biol. 2016 Jul 25. doi: 10.1038/nsmb.3265. PMID:27455459<ref>PMID:27455459</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5l7j" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 08:15, 10 August 2016
Crystal Structure of Elp3 from Dehalococcoides mccartyi
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