5dkt
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==N-terminal His tagged apPOL exonuclease mutant== | |
| + | <StructureSection load='5dkt' size='340' side='right' caption='[[5dkt]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5dkt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DKT FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5dku|5dku]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dkt OCA], [http://pdbe.org/5dkt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dkt RCSB], [http://www.ebi.ac.uk/pdbsum/5dkt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dkt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Infection by the parasite Plasmodium falciparum is the leading cause of malaria in humans. The parasite has a unique and essential plastid-like organelle called the apicoplast. The apicoplast contains a genome that undergoes replication and repair through the action of a replicative polymerase (apPOL). apPOL has no direct orthologs in mammalian polymerases and is therefore an attractive antimalarial drug target. No structural information exists for apPOL, and the Klenow fragment of Escherichia coli DNA polymerase I, which is its closest structural homolog, shares only 28% sequence identity. Here, conditions for the crystallization of and preliminary X-ray diffraction data from crystals of P. falciparum apPOL are reported. Data complete to 3.5 A resolution were collected from a single crystal (2 x 2 x 5 microm) using a 5 microm beam. The space group P6522 (unit-cell parameters a = b = 141.8, c = 149.7 A, alpha = beta = 90, gamma = 120 degrees ) was confirmed by molecular replacement. Refinement is in progress. | ||
| - | + | Crystallization and preliminary X-ray analysis of the Plasmodium falciparum apicoplast DNA polymerase.,Milton ME, Choe JY, Honzatko RB, Nelson SW Acta Crystallogr F Struct Biol Commun. 2015 Mar;71(Pt 3):333-7. doi:, 10.1107/S2053230X15002423. Epub 2015 Feb 19. PMID:25760711<ref>PMID:25760711</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5dkt" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: DNA-directed DNA polymerase]] | ||
| + | [[Category: Choe, J Y]] | ||
| + | [[Category: Honzatko, R B]] | ||
| + | [[Category: Milton, M E]] | ||
| + | [[Category: Nelson, S W]] | ||
| + | [[Category: Dna polymerase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 16:02, 10 August 2016
N-terminal His tagged apPOL exonuclease mutant
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