1nbp
From Proteopedia
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|PDB= 1nbp |SIZE=350|CAPTION= <scene name='initialview01'>1nbp</scene>, resolution 2.20Å | |PDB= 1nbp |SIZE=350|CAPTION= <scene name='initialview01'>1nbp</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=MHC:3-MERCAPTO-1-(1,3,4,9-TETRAHYDRO-B-CARBOLIN-2-YL)-PROPAN-1-ONE'>MHC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= IL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= IL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1m47|1M47]], [[1m48|1M48]], [[1m49|1M49]], [[1m4a|1M4A]], [[1m4b|1M4B]], [[1m4c|1M4C]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nbp OCA], [http://www.ebi.ac.uk/pdbsum/1nbp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nbp RCSB]</span> | ||
}} | }} | ||
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[[Category: Hyde, J.]] | [[Category: Hyde, J.]] | ||
[[Category: Randal, M.]] | [[Category: Randal, M.]] | ||
| - | [[Category: MHC]] | ||
| - | [[Category: SO4]] | ||
[[Category: cytokine]] | [[Category: cytokine]] | ||
[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
[[Category: small molecule complex]] | [[Category: small molecule complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:28:06 2008'' |
Revision as of 19:28, 30 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | IL2 (Homo sapiens) | ||||||
| Related: | 1M47, 1M48, 1M49, 1M4A, 1M4B, 1M4C
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Of Human Interleukin-2 Y31C Covalently Modified At C31 With 3-Mercapto-1-(1,3,4,9-tetrahydro-B-carbolin-2-yl)-propan-1-one
Contents |
Overview
The cytokine hormone interleukin-2 (IL-2) contains a highly adaptive region that binds small, druglike molecules. The binding properties of this adaptive region have been explored using a "tethering" method that relies on the formation of a disulfide bond between the protein and small-molecule ligands. Using tethering, surface plasmon resonance (SPR), and X-ray crystallography, we have discovered that the IL-2 adaptive region contains at least two cooperative binding sites where the binding of a first ligand to one site promotes or antagonizes the binding of a second ligand to the second site. Cooperative energies of interaction of -2 kcal/mol are observed. The observation that the adaptive region contains two adjacent sites may lead to the development of tight-binding antagonists of a protein-protein interaction. Cooperative ligand binding in the adaptive region of IL-2 underscores the importance of protein dynamics in molecular recognition. The tethering approach provides a novel and general strategy for discovering such cooperative binding interactions in specific, flexible regions of protein structure.
Disease
Known disease associated with this structure: Severe combined immunodeficiency due to IL2 deficiency OMIM:[147680]
About this Structure
1NBP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Discovery and characterization of cooperative ligand binding in the adaptive region of interleukin-2., Hyde J, Braisted AC, Randal M, Arkin MR, Biochemistry. 2003 Jun 3;42(21):6475-83. PMID:12767230
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