4pp4

From Proteopedia

(Difference between revisions)

Revision as of 07:53, 11 August 2016

Minute virus of mice non-structural protein-1N-terminal nuclease domain reveals a unique Zn2+ coordination in the active site pocket and shows a novel mode of DNA recognition at the origin of replication

Structural highlights

4pp4 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	3wrn, 3wro, 3wrq, 3wrr, 3wrs
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NS1_MUMIP] Multifunctional protein that plays an essential role in viral DNA replication. Inhibits the host cell cycle during the G1/S transition, the S-phase, and the G2/M transition. These arrests may provide essential cellular factors for viral DNA replication. Interacts specifically with the viral DNA origin of replication and plays a direct role in DNA replication. Participates in the transcriptional regulation of several promoters including the viral p38 promoter that regulates the expression of VP1 and VP2 transcripts. Promotes apoptosis in host cell.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Members of the Parvoviridae family all encode a non-structural protein 1 (NS1) that directs replication of single-stranded viral DNA, packages viral DNA into capsid, and serves as a potent transcriptional activator. Here we report the X-ray structure of the minute virus of mice (MVM) NS1 N-terminal domain at 1.45A resolution, showing that sites for dsDNA binding, ssDNA binding and cleavage, nuclear localization, and other functions are integrated on a canonical fold of the histidine-hydrophobic-histidine superfamily of nucleases, including elements specific for this Protoparvovirus but distinct from its Bocaparvovirus or Dependoparvovirus orthologs. High resolution structural analysis reveals a nickase active site with an architecture that allows highly versatile metal ligand binding. The structures support a unified mechanism of replication origin recognition for homotelomeric and heterotelomeric parvoviruses, mediated by a basic-residue-rich hairpin and an adjacent helix in the initiator proteins and by tandem tetranucleotide motifs in the replication origins.

Structures of minute virus of mice replication initiator protein N-terminal domain: Insights into DNA nicking and origin binding.,Tewary SK, Liang L, Lin Z, Lynn A, Cotmore SF, Tattersall P, Zhao H, Tang L Virology. 2014 Dec 17;476C:61-71. doi: 10.1016/j.virol.2014.11.022. PMID:25528417^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Op De Beeck A, Sobczak-Thepot J, Sirma H, Bourgain F, Brechot C, Caillet-Fauquet P. NS1- and minute virus of mice-induced cell cycle arrest: involvement of p53 and p21(cip1). J Virol. 2001 Nov;75(22):11071-8. PMID:11602746 doi:http://dx.doi.org/10.1128/JVI.75.22.11071-11078.2001
↑ Mincberg M, Gopas J, Tal J. Minute virus of mice (MVMp) infection and NS1 expression induce p53 independent apoptosis in transformed rat fibroblast cells. Virology. 2011 Mar 30;412(1):233-43. doi: 10.1016/j.virol.2010.12.035. Epub 2011 , Feb 3. PMID:21295324 doi:http://dx.doi.org/10.1016/j.virol.2010.12.035
↑ Christensen J, Cotmore SF, Tattersall P. Minute virus of mice transcriptional activator protein NS1 binds directly to the transactivation region of the viral P38 promoter in a strictly ATP-dependent manner. J Virol. 1995 Sep;69(9):5422-30. PMID:7636987
↑ Op De Beeck A, Caillet-Fauquet P. The NS1 protein of the autonomous parvovirus minute virus of mice blocks cellular DNA replication: a consequence of lesions to the chromatin? J Virol. 1997 Jul;71(7):5323-9. PMID:9188601
↑ Tewary SK, Liang L, Lin Z, Lynn A, Cotmore SF, Tattersall P, Zhao H, Tang L. Structures of minute virus of mice replication initiator protein N-terminal domain: Insights into DNA nicking and origin binding. Virology. 2014 Dec 17;476C:61-71. doi: 10.1016/j.virol.2014.11.022. PMID:25528417 doi:http://dx.doi.org/10.1016/j.virol.2014.11.022

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4pp4"

@@ Line 1: / Line 1: @@
 ==Minute virus of mice non-structural protein-1N-terminal nuclease domain reveals a unique Zn2+ coordination in the active site pocket and shows a novel mode of DNA recognition at the origin of replication==
 <StructureSection load='4pp4' size='340' side='right' caption='[[4pp4]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wrn|3wrn]], [[3wro|3wro]], [[3wrq|3wrq]], [[3wrr|3wrr]], [[3wrs|3wrs]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pp4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pp4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pp4 RCSB], [http://www.ebi.ac.uk/pdbsum/4pp4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pp4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pp4 OCA], [http://pdbe.org/4pp4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pp4 RCSB], [http://www.ebi.ac.uk/pdbsum/4pp4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pp4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4pp4" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4pp4

From Proteopedia

Revision as of 07:53, 11 August 2016

Minute virus of mice non-structural protein-1N-terminal nuclease domain reveals a unique Zn2+ coordination in the active site pocket and shows a novel mode of DNA recognition at the origin of replication

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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