1ng9
From Proteopedia
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|PDB= 1ng9 |SIZE=350|CAPTION= <scene name='initialview01'>1ng9</scene>, resolution 2.60Å | |PDB= 1ng9 |SIZE=350|CAPTION= <scene name='initialview01'>1ng9</scene>, resolution 2.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= MutS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= MutS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1e3m|1e3m]], [[1ewq|1ewq]], [[1fw6|1fw6]], [[1ewr|1ewr]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ng9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ng9 OCA], [http://www.ebi.ac.uk/pdbsum/1ng9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ng9 RCSB]</span> | ||
}} | }} | ||
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[[Category: Sixma, T K.]] | [[Category: Sixma, T K.]] | ||
[[Category: Winterwerp, H H.K.]] | [[Category: Winterwerp, H H.K.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: MG]] | ||
[[Category: abc atpase]] | [[Category: abc atpase]] | ||
[[Category: alternating atpase]] | [[Category: alternating atpase]] | ||
| Line 33: | Line 34: | ||
[[Category: dna repair]] | [[Category: dna repair]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:29:55 2008'' |
Revision as of 19:29, 30 March 2008
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| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Gene: | MutS (Escherichia coli) | ||||||
| Related: | 1e3m, 1ewq, 1fw6, 1ewr
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
E.coli MutS R697A: an ATPase-asymmetry mutant
Overview
DNA mismatch repair is an essential safeguard of genomic integrity by removing base mispairings that may arise from DNA polymerase errors or from homologous recombination between DNA strands. In Escherichia coli, the MutS enzyme recognizes mismatches and initiates repair. MutS has an intrinsic ATPase activity crucial for its function, but which is poorly understood. We show here that within the MutS homodimer, the two chemically identical ATPase sites have different affinities for ADP, and the two sites alternate in ATP hydrolysis. A single residue, Arg697, located at the interface of the two ATPase domains, controls the asymmetry. When mutated, the asymmetry is lost and mismatch repair in vivo is impaired. We propose that asymmetry of the ATPase domains is an essential feature of mismatch repair that controls the timing of the different steps in the repair cascade.
About this Structure
1NG9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The alternating ATPase domains of MutS control DNA mismatch repair., Lamers MH, Winterwerp HH, Sixma TK, EMBO J. 2003 Feb 3;22(3):746-56. PMID:12554674
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