This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1cbf
From Proteopedia
| Line 26: | Line 26: | ||
[[Category: precorrin-4 methyltransferase]] | [[Category: precorrin-4 methyltransferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15: | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:58:31 2007'' |
Revision as of 13:53, 5 November 2007
|
THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF
Overview
Biosynthesis of the corrin ring of vitamin B12 requires the action of six, S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely, related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been, determined to a resolution of 2.4 A. CbiF contains two alphabeta domains, forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The, location of AdoHcy and a number of conserved residues, helps define the, precorrin binding site. A second crystal form determined at 3.1 A, resolution highlights the flexibility of two loops around this site. CbiF, employs a unique mode of AdoHcy binding and represents a new class of, transmethylase.
About this Structure
1CBF is a Single protein structure of sequence from Bacillus megaterium with PO4 and SAH as ligands. Active as Precorrin-4 C(11)-methyltransferase, with EC number 2.1.1.133 Structure known Active Sites: , PAL and SAH. Full crystallographic information is available from OCA.
Reference
The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:9665173
Page seeded by OCA on Mon Nov 5 15:58:31 2007
