1njf
From Proteopedia
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|PDB= 1njf |SIZE=350|CAPTION= <scene name='initialview01'>1njf</scene>, resolution 2.30Å | |PDB= 1njf |SIZE=350|CAPTION= <scene name='initialview01'>1njf</scene>, resolution 2.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATG:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>ATG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span> |
|GENE= DnaX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= DnaX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1njg|1NJG]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1njf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1njf OCA], [http://www.ebi.ac.uk/pdbsum/1njf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1njf RCSB]</span> | ||
}} | }} | ||
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[[Category: Podobnik, M.]] | [[Category: Podobnik, M.]] | ||
[[Category: Weitze, T F.]] | [[Category: Weitze, T F.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: ATG]] | ||
| - | [[Category: ZN]] | ||
[[Category: aaa+ atpase domain]] | [[Category: aaa+ atpase domain]] | ||
[[Category: protein-nucleotide complex]] | [[Category: protein-nucleotide complex]] | ||
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[[Category: sensor 2]] | [[Category: sensor 2]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:31:14 2008'' |
Revision as of 19:31, 30 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | DnaX (Escherichia coli) | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Related: | 1NJG
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Nucleotide bound form of an isolated E. coli clamp loader gamma subunit
Overview
Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process.
About this Structure
1NJF is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:12623013
Page seeded by OCA on Sun Mar 30 22:31:14 2008
