1nla
From Proteopedia
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|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= ARC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= ARC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1qtg|1QTG]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nla OCA], [http://www.ebi.ac.uk/pdbsum/1nla PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nla RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NLA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1NLA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLA OCA]. |
==Reference== | ==Reference== | ||
Solution structure of switch Arc, a mutant with 3(10) helices replacing a wild-type beta-ribbon., Cordes MH, Walsh NP, McKnight CJ, Sauer RT, J Mol Biol. 2003 Feb 21;326(3):899-909. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12581649 12581649] | Solution structure of switch Arc, a mutant with 3(10) helices replacing a wild-type beta-ribbon., Cordes MH, Walsh NP, McKnight CJ, Sauer RT, J Mol Biol. 2003 Feb 21;326(3):899-909. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12581649 12581649] | ||
| + | [[Category: Enterobacteria phage p22]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Yersinia phage py54]] | ||
[[Category: Cordes, M H.]] | [[Category: Cordes, M H.]] | ||
[[Category: McKnight, C J.]] | [[Category: McKnight, C J.]] | ||
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[[Category: structural switching]] | [[Category: structural switching]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:31:59 2008'' |
Revision as of 19:32, 30 March 2008
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| Gene: | ARC (Enterobacteria phage P22) | ||||||
| Related: | 1QTG
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Switch Arc, a Mutant with 3(10) Helices Replacing a Wild-Type Beta-Ribbon
Overview
Adjacent N11L and L12N mutations in the antiparallel beta-ribbon of Arc repressor result in dramatic changes in local structure in which each beta-strand is replaced by a right-handed helix. The full solution structure of this "switch" Arc mutant shows that irregular 3(10) helices compose the new secondary structure. This structural metamorphosis conserves the number of main-chain and side-chain to main-chain hydrogen bonds and the number of fully buried core residues. Apart from a slight widening of the interhelical angle between alpha-helices A and B and changes in side-chain conformation of a few core residues in Arc, no large-scale structural adjustments in the remainder of the protein are necessary to accommodate the ribbon-to-helix change. Nevertheless, some changes in hydrogen-exchange rates are observed, even in regions that have very similar structures in the two proteins. The surface of switch Arc is packed poorly compared to wild-type, leading to approximately 1000A(2) of additional solvent-accessible surface area, and the N termini of the 3(10) helices make unfavorable head-to-head electrostatic interactions. These structural features account for the positive m value and salt dependence of the ribbon-to-helix transition in Arc-N11L, a variant that can adopt either the mutant or wild-type structures. The tertiary fold is capped in different ways in switch and wild-type Arc, showing how stepwise evolutionary transformations can arise through small changes in amino acid sequence.
About this Structure
1NLA is a Single protein structure of sequence from Enterobacteria phage p22. Full crystallographic information is available from OCA.
Reference
Solution structure of switch Arc, a mutant with 3(10) helices replacing a wild-type beta-ribbon., Cordes MH, Walsh NP, McKnight CJ, Sauer RT, J Mol Biol. 2003 Feb 21;326(3):899-909. PMID:12581649
Page seeded by OCA on Sun Mar 30 22:31:59 2008
