This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4hfn
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | |||
==X-ray Crystal Structure of a Ternary Complex of Double Bond Reductase from Nicotiana tabacum== | ==X-ray Crystal Structure of a Ternary Complex of Double Bond Reductase from Nicotiana tabacum== | ||
<StructureSection load='4hfn' size='340' side='right' caption='[[4hfn]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='4hfn' size='340' side='right' caption='[[4hfn]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[4hfn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[4hfn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HFN FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIY:(2E)-3-(4-HYDROXY-3-METHOXYPHENYL)PROP-2-ENAL'>CIY</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIY:(2E)-3-(4-HYDROXY-3-METHOXYPHENYL)PROP-2-ENAL'>CIY</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hfj|4hfj]], [[4hfm|4hfm]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hfj|4hfj]], [[4hfm|4hfm]]</td></tr> | ||
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NtADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NtADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2- | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-alkenal_reductase_(NAD(P)(+)) 2-alkenal reductase (NAD(P)(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.74 1.3.1.74] </span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hfn RCSB], [http://www.ebi.ac.uk/pdbsum/4hfn PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfn OCA], [http://pdbe.org/4hfn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hfn RCSB], [http://www.ebi.ac.uk/pdbsum/4hfn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfn ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[http://www.uniprot.org/uniprot/ | + | [[http://www.uniprot.org/uniprot/DBR_TOBAC DBR_TOBAC]] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3] |
==See Also== | ==See Also== | ||
| Line 18: | Line 19: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: American tobacco]] |
| - | + | ||
[[Category: Scrutton, N S]] | [[Category: Scrutton, N S]] | ||
[[Category: Toogood, H S]] | [[Category: Toogood, H S]] | ||
Revision as of 11:22, 11 August 2016
X-ray Crystal Structure of a Ternary Complex of Double Bond Reductase from Nicotiana tabacum
| |||||||||||
