This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4l40

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 12:25, 11 August 2016

Structure of the P450 OleT with a C20 fatty acid substrate bound

Structural highlights

4l40 is a 1 chain structure with sequence from Jeotgalicoccus sp. atcc 8456. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4l54
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The production of hydrocarbons in nature has been documented for only a limited set of organisms, with many of the molecular components underpinning these processes only recently identified. There is an obvious scope for application of these catalysts and engineered variants thereof in the future production of biofuels. Here we present biochemical characterization and crystal structures of a cytochrome P450 fatty acid peroxygenase: the terminal alkene forming OleTJE (CYP152L1) from Jeotgalicoccus sp. 8456. OleTJE is stabilized at high ionic strength, but aggregation and precipitation of OleTJE in low salt buffer can be turned to advantage for purification, because resolubilized OleTJE is fully active and extensively dissociated from lipids. OleTJE binds avidly to a range of long chain fatty acids, and structures of both ligand-free and arachidic acid-bound OleTJE reveal that the P450 active site is preformed for fatty acid binding. OleTJE heme iron has an unusually positive redox potential (-103 mV versus normal hydrogen electrode), which is not significantly affected by substrate binding, despite extensive conversion of the heme iron to a high spin ferric state. Terminal alkenes are produced from a range of saturated fatty acids (C12-C20), and stopped-flow spectroscopy indicates a rapid reaction between peroxide and fatty acid-bound OleTJE (167 s(-1) at 200 mum H2O2). Surprisingly, the active site is highly similar in structure to the related P450BSbeta, which catalyzes hydroxylation of fatty acids as opposed to decarboxylation. Our data provide new insights into structural and mechanistic properties of a robust P450 with potential industrial applications.

Structure and Biochemical Properties of the Alkene Producing Cytochrome P450 OleTJE (CYP152L1) from the Jeotgalicoccus sp. 8456 Bacterium.,Belcher J, McLean KJ, Matthews S, Woodward LS, Fisher K, Rigby SE, Nelson DR, Potts D, Baynham MT, Parker DA, Leys D, Munro AW J Biol Chem. 2014 Mar 7;289(10):6535-50. doi: 10.1074/jbc.M113.527325. Epub 2014 , Jan 18. PMID:24443585^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Belcher J, McLean KJ, Matthews S, Woodward LS, Fisher K, Rigby SE, Nelson DR, Potts D, Baynham MT, Parker DA, Leys D, Munro AW. Structure and Biochemical Properties of the Alkene Producing Cytochrome P450 OleTJE (CYP152L1) from the Jeotgalicoccus sp. 8456 Bacterium. J Biol Chem. 2014 Mar 7;289(10):6535-50. doi: 10.1074/jbc.M113.527325. Epub 2014 , Jan 18. PMID:24443585 doi:http://dx.doi.org/10.1074/jbc.M113.527325

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4l40"

@@ Line 1: / Line 1: @@
 ==Structure of the P450 OleT with a C20 fatty acid substrate bound==
 <StructureSection load='4l40' size='340' side='right' caption='[[4l40]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DCR:ICOSANOIC+ACID'>DCR</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4l54|4l54]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l40 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l40 RCSB], [http://www.ebi.ac.uk/pdbsum/4l40 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l40 OCA], [http://pdbe.org/4l40 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l40 RCSB], [http://www.ebi.ac.uk/pdbsum/4l40 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l40 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4l40" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4l40

From Proteopedia

Revision as of 12:25, 11 August 2016

Structure of the P450 OleT with a C20 fatty acid substrate bound

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox