4crw

From Proteopedia

(Difference between revisions)

Revision as of 14:45, 11 August 2016

Complex of human DDX6 (RECA-C) and CNOT1 (MIF4G)

Structural highlights

4crw is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4gml, 4gmj, 2wax, 2way
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[DDX6_HUMAN] Note=A chromosomal aberration involving DDX6 may be a cause of hematopoietic tumors such as B-cell lymphomas. Translocation t(11;14)(q23;q32).

Function

[CNOT1_HUMAN] Belongs to the CCR4-NOT complex that functions as general transcription regulation complex. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors.^[1] ^[2] [DDX6_HUMAN] In the process of mRNA degradation, may play a role in mRNA decapping.

Publication Abstract from PubMed

CCR4-NOT is a major effector complex in miRNA-mediated gene silencing. It is recruited to miRNA targets through interactions with tryptophan (W)-containing motifs in TNRC6/GW182 proteins and is required for both translational repression and degradation of miRNA targets. Here, we elucidate the structural basis for the repressive activity of CCR4-NOT and its interaction with TNRC6/GW182s. We show that the conserved CNOT9 subunit attaches to a domain of unknown function (DUF3819) in the CNOT1 scaffold. The resulting complex provides binding sites for TNRC6/GW182, and its crystal structure reveals tandem W-binding pockets located in CNOT9. We further show that the CNOT1 MIF4G domain interacts with the C-terminal RecA domain of DDX6, a translational repressor and decapping activator. The crystal structure of this complex demonstrates striking similarity to the eIF4G-eIF4A complex. Together, our data provide the missing physical links in a molecular pathway that connects miRNA target recognition with translational repression, deadenylation, and decapping.

A DDX6-CNOT1 Complex and W-Binding Pockets in CNOT9 Reveal Direct Links between miRNA Target Recognition and Silencing.,Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E Mol Cell. 2014 Apr 22. pii: S1097-2765(14)00267-6. doi:, 10.1016/j.molcel.2014.03.034. PMID:24768540^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT. Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. Nucleic Acids Res. 2000 Feb 1;28(3):809-17. PMID:10637334
↑ Winkler GS, Mulder KW, Bardwell VJ, Kalkhoven E, Timmers HT. Human Ccr4-Not complex is a ligand-dependent repressor of nuclear receptor-mediated transcription. EMBO J. 2006 Jul 12;25(13):3089-99. Epub 2006 Jun 15. PMID:16778766 doi:7601194
↑ Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E. A DDX6-CNOT1 Complex and W-Binding Pockets in CNOT9 Reveal Direct Links between miRNA Target Recognition and Silencing. Mol Cell. 2014 Apr 22. pii: S1097-2765(14)00267-6. doi:, 10.1016/j.molcel.2014.03.034. PMID:24768540 doi:http://dx.doi.org/10.1016/j.molcel.2014.03.034

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4crw"

@@ Line 1: / Line 1: @@
 ==Complex of human DDX6 (RECA-C) and CNOT1 (MIF4G)==
 <StructureSection load='4crw' size='340' side='right' caption='[[4crw]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gml|4gml]], [[4gmj|4gmj]], [[2wax|2wax]], [[2way|2way]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4crw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4crw RCSB], [http://www.ebi.ac.uk/pdbsum/4crw PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4crw OCA], [http://pdbe.org/4crw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4crw RCSB], [http://www.ebi.ac.uk/pdbsum/4crw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4crw ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 19: / Line 20: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4crw" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4crw

From Proteopedia

Revision as of 14:45, 11 August 2016

Complex of human DDX6 (RECA-C) and CNOT1 (MIF4G)

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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