1nvm
From Proteopedia
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|PDB= 1nvm |SIZE=350|CAPTION= <scene name='initialview01'>1nvm</scene>, resolution 1.70Å | |PDB= 1nvm |SIZE=350|CAPTION= <scene name='initialview01'>1nvm</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] </span> |
|GENE= DMPG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.]), DMPF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.]) | |GENE= DMPG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.]), DMPF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nvm OCA], [http://www.ebi.ac.uk/pdbsum/1nvm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nvm RCSB]</span> | ||
}} | }} | ||
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[[Category: Powlowski, J.]] | [[Category: Powlowski, J.]] | ||
[[Category: Vrielink, A.]] | [[Category: Vrielink, A.]] | ||
| - | [[Category: MN]] | ||
| - | [[Category: MPD]] | ||
| - | [[Category: NAD]] | ||
| - | [[Category: OXL]] | ||
| - | [[Category: SO4]] | ||
[[Category: bifunctional enzyme]] | [[Category: bifunctional enzyme]] | ||
[[Category: sequestered tunnel]] | [[Category: sequestered tunnel]] | ||
[[Category: substrate channeling]] | [[Category: substrate channeling]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:36:06 2008'' |
Revision as of 19:36, 30 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Gene: | DMPG (Pseudomonas sp.), DMPF (Pseudomonas sp.) | ||||||
| Activity: | Acetaldehyde dehydrogenase (acetylating), with EC number 1.2.1.10 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a bifunctional aldolase-dehydrogenase : sequestering a reactive and volatile intermediate
Overview
The crystal structure of the bifunctional enzyme 4-hydroxy-2-ketovalerate aldolase (DmpG)/acylating acetaldehyde dehydrogenase (DmpF), which is involved in the bacterial degradation of toxic aromatic compounds, has been determined by multiwavelength anomalous dispersion (MAD) techniques and refined to 1.7-A resolution. Structures of the two polypeptides represent a previously unrecognized subclass of metal-dependent aldolases, and of a CoA-dependent dehydrogenase. The structure reveals a mixed state of NAD+ binding to the DmpF protomer. Domain movements associated with cofactor binding in the DmpF protomer may be correlated with channeling and activity at the DmpG protomer. In the presence of NAD+ a 29-A-long sequestered tunnel links the two active sites. Two barriers are visible along the tunnel and suggest control points for the movement of the reactive and volatile acetaldehyde intermediate between the two active sites.
About this Structure
1NVM is a Protein complex structure of sequences from Pseudomonas sp.. Full crystallographic information is available from OCA.
Reference
Crystal structure of a bifunctional aldolase-dehydrogenase: sequestering a reactive and volatile intermediate., Manjasetty BA, Powlowski J, Vrielink A, Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6992-7. Epub 2003 May 22. PMID:12764229
Page seeded by OCA on Sun Mar 30 22:36:06 2008
