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4w70

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Revision as of 16:18, 11 August 2016

Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 7.0

Structural highlights

4w70 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4tyu, 4tys, 4u05, 4w68
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Camelid single domain antibodies (sdAb) are known for their thermal stability and reversible refolding. We have characterized an unusually stable sdAb recognizing Staphylococcal enterotoxin B with one of the highest reported melting temperatures (Tm = 85 degrees C). Unexpectedly, ~10-20% of the protein formed a dimer in solution. Three other cases where <20% of the sdAb dimerized have been reported; however, this is the first report of both the monomeric and dimeric X-ray crystal structures. Concentration of the monomer did not lead to the formation of new dimer suggesting a stable conformationally distinct species in a fraction of the cytoplasmically expressed protein. Comparison of periplasmic and cytoplasmic expression showed that the dimer was associated with cytoplasmic expression. The disulfide bond was partially reduced in the WT protein purified from the cytoplasm and the protein irreversibly unfolded. Periplasmic expression produced monomeric protein with a fully formed disulfide bond and mostly reversible refolding. Crystallization of a disulfide-bond free variant, C22A/C99A, purified from the periplasm yielded a structure of a monomeric form, while crystallization of C22A/C99A from the cytoplasm produced an asymmetric dimer. In the dimer, a significant conformational asymmetry was found in the loop residues of the edge beta-strands (S50-Y60) containing the highly variable complementarity determining region, CDR2. Two dimeric assemblies were predicted from the crystal packing. Mutation of a residue at one of the interfaces, Y98A, disrupted the dimer in solution. The pleomorphic homodimer may yield insight into the stability of misfolded states and the importance of the conserved disulfide bond in preventing their formation. (c) Proteins 2014;. (c) 2014 Wiley Periodicals, Inc.

Structural and mutational analysis of a monomeric and dimeric form of a single domain antibody with implications for protein misfolding.,George J, Compton JR, Leary DH, Olson MA, Legler PM Proteins. 2014 Aug 18. doi: 10.1002/prot.24671. PMID:25136772^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ George J, Compton JR, Leary DH, Olson MA, Legler PM. Structural and mutational analysis of a monomeric and dimeric form of a single domain antibody with implications for protein misfolding. Proteins. 2014 Aug 18. doi: 10.1002/prot.24671. PMID:25136772 doi:http://dx.doi.org/10.1002/prot.24671

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4w70"

Categories: George, J | Legler, P M | Single domain antibody | V-set domain

@@ Line 1: / Line 1: @@
 ==Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 7.0==
 <StructureSection load='4w70' size='340' side='right' caption='[[4w70]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tyu|4tyu]], [[4tys|4tys]], [[4u05|4u05]], [[4w68|4w68]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w70 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4w70 RCSB], [http://www.ebi.ac.uk/pdbsum/4w70 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4w70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w70 OCA], [http://pdbe.org/4w70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4w70 RCSB], [http://www.ebi.ac.uk/pdbsum/4w70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4w70 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4w70" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4w70

From Proteopedia

Revision as of 16:18, 11 August 2016

Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 7.0

Structural highlights

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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