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1nzs
From Proteopedia
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|PDB= 1nzs |SIZE=350|CAPTION= <scene name='initialview01'>1nzs</scene> | |PDB= 1nzs |SIZE=350|CAPTION= <scene name='initialview01'>1nzs</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nzs OCA], [http://www.ebi.ac.uk/pdbsum/1nzs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nzs RCSB]</span> | ||
}} | }} | ||
| Line 33: | Line 36: | ||
[[Category: signal termination]] | [[Category: signal termination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:37:48 2008'' |
Revision as of 19:37, 30 March 2008
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state
Overview
Visual arrestin binds to the phosphorylated carboxy-terminal region of rhodopsin to block interactions with transducin and terminate signaling in the rod photoreceptor cells. A synthetic seven-phospho-peptide from the C-terminal region of rhodopsin, Rh(330-348), has been shown to bind arrestin and mimic inhibition of signal transduction. In this study, we examine conformational changes in this synthetic peptide upon binding to arrestin by high-resolution proton nuclear magnetic resonance (NMR). We show that the peptide is completely disordered in solution, but becomes structured upon binding to arrestin. A control, unphosphorylated peptide that fails to bind to arrestin remains highly disordered. Specific NMR distance constraints are used to model the arrestin-bound conformation. The models suggest that the phosphorylated carboxy-terminal region of rhodopsin, Rh(330-348), undergoes significant conformational changes and becomes structured upon binding to arrestin.
About this Structure
1NZS is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
The arrestin-bound conformation and dynamics of the phosphorylated carboxy-terminal region of rhodopsin., Kisselev OG, McDowell JH, Hargrave PA, FEBS Lett. 2004 Apr 30;564(3):307-11. PMID:15111114
Page seeded by OCA on Sun Mar 30 22:37:48 2008
