1nzv
From Proteopedia
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|PDB= 1nzv |SIZE=350|CAPTION= <scene name='initialview01'>1nzv</scene>, resolution 2.10Å | |PDB= 1nzv |SIZE=350|CAPTION= <scene name='initialview01'>1nzv</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] </span> |
|GENE= V-SRC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus]) | |GENE= V-SRC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1iso|1ISO]], [[1kc2|1KC2]], [[1nzl|1NZL]], [[1sps|1SPS]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nzv OCA], [http://www.ebi.ac.uk/pdbsum/1nzv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nzv RCSB]</span> | ||
}} | }} | ||
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[[Category: Lubman, O Y.]] | [[Category: Lubman, O Y.]] | ||
[[Category: Waksman, G.]] | [[Category: Waksman, G.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: PG4]] | ||
[[Category: phosphotyrosine]] | [[Category: phosphotyrosine]] | ||
[[Category: sh2 domain]] | [[Category: sh2 domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:37:51 2008'' |
Revision as of 19:37, 30 March 2008
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| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | V-SRC (Rous sarcoma virus) | ||||||
| Activity: | Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 | ||||||
| Related: | 1ISO, 1KC2, 1NZL, 1SPS
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Src SH2 domain bound to doubly phosphorylated peptide PQpYIpYVPA
Overview
Recruitment of the Src kinase to the activated form of the platelet-derived growth factor (PDGF) receptor involves recognition of a unique sequence motif in the juxtamembrane region of the receptor by the Src homology 2 (SH2) domain of the enzyme. This motif contains two phosphotyrosine residues separated by one residue (sequence pYIpYV where pY indicates a phosphotyrosine). Here, we provide the thermodynamic and structural basis for the binding of this motif by the Src SH2 domain. We show that the second phosphorylation event increases the free energy window for specific interaction and that the physiological target is exquisitely designed for the task of recruiting specifically an SH2 domain which otherwise demonstrates very little intrinsic ability to discriminate sequences C-terminal to the first phosphorylation event. Surprisingly, we show that water plays a role in the recognition process.
About this Structure
1NZV is a Single protein structure of sequence from Rous sarcoma virus. Full crystallographic information is available from OCA.
Reference
Structural and thermodynamic basis for the interaction of the Src SH2 domain with the activated form of the PDGF beta-receptor., Lubman OY, Waksman G, J Mol Biol. 2003 May 2;328(3):655-68. PMID:12706723
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