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4q9b

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Revision as of 17:40, 11 August 2016

IgNAR antibody domain C2

Structural highlights

4q9b is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1rei, 4q97, 4q9c
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Sharks and other cartilaginous fish are the phylogenetically oldest living organisms that rely on antibodies as part of their adaptive immune system. They produce the immunoglobulin new antigen receptor (IgNAR), a homodimeric heavy chain-only antibody, as a major part of their humoral adaptive immune response. Here, we report the atomic resolution structure of the IgNAR constant domains and a structural model of this heavy chain-only antibody. We find that despite low sequence conservation, the basic Ig fold of modern antibodies is already present in the evolutionary ancient shark IgNAR domains, highlighting key structural determinants of the ubiquitous Ig fold. In contrast, structural differences between human and shark antibody domains explain the high stability of several IgNAR domains and allowed us to engineer human antibodies for increased stability and secretion efficiency. We identified two constant domains, C1 and C3, that act as dimerization modules within IgNAR. Together with the individual domain structures and small-angle X-ray scattering, this allowed us to develop a structural model of the complete IgNAR molecule. Its constant region exhibits an elongated shape with flexibility and a characteristic kink in the middle. Despite the lack of a canonical hinge region, the variable domains are spaced appropriately wide for binding to multiple antigens. Thus, the shark IgNAR domains already display the well-known Ig fold, but apart from that, this heavy chain-only antibody employs unique ways for dimerization and positioning of functional modules.

The structural analysis of shark IgNAR antibodies reveals evolutionary principles of immunoglobulins.,Feige MJ, Grawert MA, Marcinowski M, Hennig J, Behnke J, Auslander D, Herold EM, Peschek J, Castro CD, Flajnik M, Hendershot LM, Sattler M, Groll M, Buchner J Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8155-60. doi:, 10.1073/pnas.1321502111. Epub 2014 May 15. PMID:24830426^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Feige MJ, Grawert MA, Marcinowski M, Hennig J, Behnke J, Auslander D, Herold EM, Peschek J, Castro CD, Flajnik M, Hendershot LM, Sattler M, Groll M, Buchner J. The structural analysis of shark IgNAR antibodies reveals evolutionary principles of immunoglobulins. Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8155-60. doi:, 10.1073/pnas.1321502111. Epub 2014 May 15. PMID:24830426 doi:http://dx.doi.org/10.1073/pnas.1321502111

1 Structural highlights
2 Publication Abstract from PubMed
3 References

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Retrieved from "http://52.214.119.220/wiki/index.php/4q9b"

@@ Line 1: / Line 1: @@
 ==IgNAR antibody domain C2==
 <StructureSection load='4q9b' size='340' side='right' caption='[[4q9b]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4q9b]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q9B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q9B FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rei|1rei]], [[4q97|4q97]], [[4q9c|4q9c]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q9b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q9b RCSB], [http://www.ebi.ac.uk/pdbsum/4q9b PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q9b OCA], [http://pdbe.org/4q9b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q9b RCSB], [http://www.ebi.ac.uk/pdbsum/4q9b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q9b ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 14: / Line 15: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4q9b" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4q9b

From Proteopedia

Revision as of 17:40, 11 August 2016

IgNAR antibody domain C2

Structural highlights

Publication Abstract from PubMed

References

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