1ol2
From Proteopedia
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|PDB= 1ol2 |SIZE=350|CAPTION= <scene name='initialview01'>1ol2</scene>, resolution 2.6Å | |PDB= 1ol2 |SIZE=350|CAPTION= <scene name='initialview01'>1ol2</scene>, resolution 2.6Å | ||
|SITE= <scene name='pdbsite=CBB:Cyclin+Binding+Groove+Chain+D'>CBB</scene> | |SITE= <scene name='pdbsite=CBB:Cyclin+Binding+Groove+Chain+D'>CBB</scene> | ||
| - | |LIGAND= <scene name='pdbligand=NH2:AMINO GROUP'>NH2</scene> | + | |LIGAND= <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PFF:4-FLUORO-L-PHENYLALANINE'>PFF</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/ | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ol2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ol2 OCA], [http://www.ebi.ac.uk/pdbsum/1ol2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ol2 RCSB]</span> | ||
}} | }} | ||
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Insights into cyclin groove recognition: complex crystal structures and inhibitor design through ligand exchange., Kontopidis G, Andrews MJ, McInnes C, Cowan A, Powers H, Innes L, Plater A, Griffiths G, Paterson D, Zheleva DI, Lane DP, Green S, Walkinshaw MD, Fischer PM, Structure. 2003 Dec;11(12):1537-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14656438 14656438] | Insights into cyclin groove recognition: complex crystal structures and inhibitor design through ligand exchange., Kontopidis G, Andrews MJ, McInnes C, Cowan A, Powers H, Innes L, Plater A, Griffiths G, Paterson D, Zheleva DI, Lane DP, Green S, Walkinshaw MD, Fischer PM, Structure. 2003 Dec;11(12):1537-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14656438 14656438] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| + | [[Category: Non-specific serine/threonine protein kinase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Transferred entry: 2 7.11 1]] | ||
[[Category: Andrews, M.]] | [[Category: Andrews, M.]] | ||
[[Category: Cowan, A.]] | [[Category: Cowan, A.]] | ||
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[[Category: Walkinshaw, M.]] | [[Category: Walkinshaw, M.]] | ||
[[Category: Zheleva, D.]] | [[Category: Zheleva, D.]] | ||
| - | [[Category: NH2]] | ||
[[Category: cyclin some]] | [[Category: cyclin some]] | ||
[[Category: drug design]] | [[Category: drug design]] | ||
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[[Category: peptidomimetic]] | [[Category: peptidomimetic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:46:50 2008'' |
Revision as of 19:46, 30 March 2008
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| , resolution 2.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , | ||||||
| Activity: | Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CYCLIN A BINDING GROOVE INHIBITOR H-ARG-ARG-LEU-ASN-(P-F-PHE)-NH2
Overview
Inhibition of CDK2/CA (cyclin-dependent kinase 2/cyclin A complex) activity through blocking of the substrate recognition site in the cyclin A subunit has been demonstrated to be an effective method for inducing apoptosis in tumor cells. We have used the cyclin binding motif (CBM) present in the tumor suppressor proteins p21(WAF1) and p27(KIP1) as a template to optimize the minimal sequence necessary for CDK2/CA inhibition. A series of peptides were prepared, containing nonnatural amino acids, which possess nano- to micromolar CDK2-inhibitory activity. Here we present X-ray structures of the protein complex CDK2/CA, together with the cyclin groove-bound peptides H-Ala-Ala-Abu-Arg-Ser-Leu-Ile-(p-F-Phe)-NH(2) (peptide 1), H-Arg-Arg-Leu-Ile-Phe-NH(2) (peptide 2), Ac-Arg-Arg-Leu-Asn-(m-Cl-Phe)-NH(2) (peptide 3), H-Arg-Arg-Leu-Asn-(p-F-Phe)-NH(2) (peptide 4), and H-Cit-Cit-Leu-Ile-(p-F-Phe)-NH(2) (peptide 5). Some of the peptide complexes presented here were obtained through the novel technique of ligand exchange within protein crystals. This method may find general application for obtaining complex structures of proteins with surface-bound ligands.
About this Structure
1OL2 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Insights into cyclin groove recognition: complex crystal structures and inhibitor design through ligand exchange., Kontopidis G, Andrews MJ, McInnes C, Cowan A, Powers H, Innes L, Plater A, Griffiths G, Paterson D, Zheleva DI, Lane DP, Green S, Walkinshaw MD, Fischer PM, Structure. 2003 Dec;11(12):1537-46. PMID:14656438
Page seeded by OCA on Sun Mar 30 22:46:50 2008
Categories: Homo sapiens | Non-specific serine/threonine protein kinase | Protein complex | Andrews, M. | Cowan, A. | Fischer, P. | Green, S. | Griffiths, G. | Innes, L. | Kontopidis, G. | Lane, D. | Mcinnes, C. | Paterson, D. | Plater, A. | Powers, H. | Walkinshaw, M. | Zheleva, D. | Cyclin some | Drug design | Inhibitor | Kinase | Ligand exchange | Peptidomimetic
