1on1
From Proteopedia
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|PDB= 1on1 |SIZE=350|CAPTION= <scene name='initialview01'>1on1</scene>, resolution 1.75Å | |PDB= 1on1 |SIZE=350|CAPTION= <scene name='initialview01'>1on1</scene>, resolution 1.75Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1on2|1ON2]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1on1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1on1 OCA], [http://www.ebi.ac.uk/pdbsum/1on1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1on1 RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Guedon, E.]] | [[Category: Guedon, E.]] | ||
[[Category: Helmann, J D.]] | [[Category: Helmann, J D.]] | ||
| - | [[Category: MN]] | ||
[[Category: dna-binding protein]] | [[Category: dna-binding protein]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
[[Category: metalloregulatory protein]] | [[Category: metalloregulatory protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:47:32 2008'' |
Revision as of 19:47, 30 March 2008
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| , resolution 1.75Å | |||||||
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| Ligands: | |||||||
| Related: | 1ON2
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound To Manganese, AB Conformation.
Overview
The Bacillus subtilis manganese transport regulator, MntR, binds Mn2+ as an effector and is a repressor of transporters that import manganese. A member of the diphtheria toxin repressor (DtxR) family of metalloregulatory proteins, MntR exhibits selectivity for Mn2+ over Fe2+. Replacement of a metal-binding residue, Asp8, with methionine (D8M) relaxes this specificity. We report here the X-ray crystal structures of wild-type MntR and the D8M mutant bound to manganese with 1.75 A and 1.61 A resolution, respectively. The 142-residue MntR homodimer has substantial structural similarity to the 226-residue DtxR but lacks the C-terminal SH3-like domain of DtxR. The metal-binding pockets of MntR and DtxR are substantially different. The cation-to-cation distance between the two manganese ions bound by MntR is 3.3 A, whereas that between the metal ions bound by DtxR is 9 A. D8M binds only a single Mn2+ per monomer, owing to alteration of the metal-binding site. The sole retained metal site adopts pseudo-hexacoordinate geometry rather than the pseudo-heptacoordinate geometry of the MntR metal sites.
About this Structure
1ON1 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Structure of the manganese-bound manganese transport regulator of Bacillus subtilis., Glasfeld A, Guedon E, Helmann JD, Brennan RG, Nat Struct Biol. 2003 Aug;10(8):652-7. PMID:12847518
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