1onj
From Proteopedia
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|PDB= 1onj |SIZE=350|CAPTION= <scene name='initialview01'>1onj</scene>, resolution 1.555Å | |PDB= 1onj |SIZE=350|CAPTION= <scene name='initialview01'>1onj</scene>, resolution 1.555Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1onj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1onj OCA], [http://www.ebi.ac.uk/pdbsum/1onj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1onj RCSB]</span> | ||
}} | }} | ||
| Line 32: | Line 35: | ||
[[Category: Tu, X.]] | [[Category: Tu, X.]] | ||
[[Category: Xu, C.]] | [[Category: Xu, C.]] | ||
| - | [[Category: SO4]] | ||
[[Category: beta-turn-beta]] | [[Category: beta-turn-beta]] | ||
[[Category: three-finger protein]] | [[Category: three-finger protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:47:46 2008'' |
Revision as of 19:47, 30 March 2008
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| , resolution 1.555Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Atratoxin-b from Chinese cobra venom of Naja atra
Overview
Atratoxin-b, a short-chain alpha-neurotoxin purified from Naja atra (mainland Chinese cobra) venom using a three-step chromatography procedure, has an apparent molecular mass of 6950 Da with an alkaline pI value (>9.5) and consists of one single polypeptide chain as estimated by MALDI-TOF mass spectrometry and SDS-PAGE. The protein is toxic to mice, with an in vitro LD(50) of about 0.18 mg kg(-1). Its N-terminal amino-acid sequence, LECHNQQSSQTPTIT, displays a very high homology to those of other alpha-neurotoxins. The overall three-dimensional structure of atratoxin-b is very similar to that of the homologous erabutoxin-a, as shown by the crystallographic molecular replacement and preliminary refinement results, with an R factor and R(free) of 27 and 29%, respectively. The microcrystal slowly grew to dimensions of approximate 0.1 x 0.1 x 0.15 mm over eight months using hanging-drop vapour-diffusion method. It gave a set of diffraction data to 1.56 A resolution using X-rays of wavelength 1.1516 A generated by the X-ray Diffraction and Scattering Station of beamline U7B at the National Synchrotron Radiation Laboratory (Hefei, China); this is the first example of the use of this beamline in protein crystallography. The crystals belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = 49.28, c = 44.80 A, corresponding to one molecule per asymmetric unit and a volume-to-mass ratio of 1.96 A(3) Da(-1).
About this Structure
1ONJ is a Single protein structure of sequence from Naja atra. Full crystallographic information is available from OCA.
Reference
Purification, N-terminal sequencing, crystallization and preliminary structural determination of atratoxin-b, a short-chain alpha-neurotoxin from Naja atra venom., Lou X, Tu X, Pan G, Xu C, Fan R, Lu W, Deng W, Rao P, Teng M, Niu L, Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1038-42. Epub 2003, May 23. PMID:12777767
Page seeded by OCA on Sun Mar 30 22:47:46 2008
Categories: Naja atra | Single protein | Deng, W. | Fan, R. | Lou, X. | Lu, W. | Niu, L. | Pan, G. | Rao, P. | Teng, M. | Tu, X. | Xu, C. | Beta-turn-beta | Three-finger protein
