1oo3
From Proteopedia
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1oo4|1OO4]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oo3 OCA], [http://www.ebi.ac.uk/pdbsum/1oo3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oo3 RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: src homology 2 domain p85 regulatory subunit mutant]] | [[Category: src homology 2 domain p85 regulatory subunit mutant]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:48:06 2008'' |
Revision as of 19:48, 30 March 2008
| |||||||
| Related: | 1OO4
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase
Overview
Understanding the specificity of Src homology 2 (SH2) domains is important because of their critical role in cell signaling. Previous genetic analysis has characterized mutants of the N-terminal src homology 2 (SH2) domain of the p85 subunit of phosphoinositide 3-kinase (PI3K). The P395S mutant exhibits a specificity for phosphopeptide binding different from that of the wild-type SH2. The P395S mutant has an increased affinity for the platelet-derived growth factor receptor (PDGFr) compared to polyomavirus middle T antigen (MT). Solution structures of the P395S mutant of the p85 N-SH2 alone and complexed to a PDGFr phosphopeptide were determined to explain the change in specificity. Chemical shift perturbations caused by different peptides were compared for mutant and wild-type structures. The results show that the single P395S mutation has broad effects on the structure. Furthermore, they provide a rationale for the observed changes in binding preference.
About this Structure
1OO3 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Nuclear magnetic resonance structure of the P395S mutant of the N-SH2 domain of the p85 subunit of PI3 kinase: an SH2 domain with altered specificity., Gunther UL, Weyrauch B, Zhang X, Schaffhausen B, Biochemistry. 2003 Sep 30;42(38):11120-7. PMID:14503862
Page seeded by OCA on Sun Mar 30 22:48:06 2008
