1os0
From Proteopedia
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|PDB= 1os0 |SIZE=350|CAPTION= <scene name='initialview01'>1os0</scene>, resolution 2.10Å | |PDB= 1os0 |SIZE=350|CAPTION= <scene name='initialview01'>1os0</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=DPH:DEAMINO-METHYL-PHENYLALANINE'>DPH</scene>, <scene name='pdbligand=PPH:PHENYLALANINE+PHOSPHINIC+ACID'>PPH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1kei|1KEI]], [[2tmn|2TMN]], [[1qf0|1QF0]], [[1qf1|1QF1]], [[1qf2|1QF2]], [[1tlp|1TLP]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1os0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1os0 OCA], [http://www.ebi.ac.uk/pdbsum/1os0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1os0 RCSB]</span> | ||
}} | }} | ||
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[[Category: Selkti, M.]] | [[Category: Selkti, M.]] | ||
[[Category: Tomas, A.]] | [[Category: Tomas, A.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: DMS]] | ||
| - | [[Category: PPH]] | ||
| - | [[Category: ZN]] | ||
[[Category: alpha-amino phosphinic compound]] | [[Category: alpha-amino phosphinic compound]] | ||
[[Category: inhibition]] | [[Category: inhibition]] | ||
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[[Category: thermolysin]] | [[Category: thermolysin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:29 2008'' |
Revision as of 19:49, 30 March 2008
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| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Activity: | Thermolysin, with EC number 3.4.24.27 | ||||||
| Related: | 1KEI, 2TMN, 1QF0, 1QF1, 1QF2, 1TLP
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THERMOLYSIN WITH AN ALPHA-AMINO PHOSPHINIC INHIBITOR
Overview
A new alpha-aminophosphinic compound able to inhibit both zinc-containing exopeptidases and endopeptidases has been crystallized with TLN as a model in order to investigate the mode of zinc recognition by the phosphinic moiety and to evaluate the potential role of the free alpha-amino group in the formation of enzyme-inhibitor complexes. In addition to the main interactions between the backbone of the inhibitor and the enzyme active site, it is observed that the phosphinic group acts as a distorted bidentate ligand for the zinc ion, while the free alpha-amino function does not directly participate in interactions within the active site. Association of the present data and the K(i) values of various analogues of the inhibitor towards TLN and neprilysin suggests differences in the hydrophobicity of the S(1)-S(2) domains of the enzymes. This could be taken into account in the design of selective inhibitors.
About this Structure
1OS0 is a Single protein structure of sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA.
Reference
Interactions of a new alpha-aminophosphinic derivative inside the active site of TLN (thermolysin): a model for zinc-metalloendopeptidase inhibition., Selkti M, Tomas A, Gaucher JF, Prange T, Fournie-Zaluski MC, Chen H, Roques BP, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1200-5. Epub 2003, Jun 27. PMID:12832763
Page seeded by OCA on Sun Mar 30 22:49:29 2008
