1ou8
From Proteopedia
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|PDB= 1ou8 |SIZE=350|CAPTION= <scene name='initialview01'>1ou8</scene>, resolution 1.60Å | |PDB= 1ou8 |SIZE=350|CAPTION= <scene name='initialview01'>1ou8</scene>, resolution 1.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= SSPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae]) | |GENE= SSPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ou9|1OU9]], [[1oul|1OUL]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ou8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ou8 OCA], [http://www.ebi.ac.uk/pdbsum/1ou8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ou8 RCSB]</span> | ||
}} | }} | ||
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[[Category: Sauer, R T.]] | [[Category: Sauer, R T.]] | ||
[[Category: Wah, D A.]] | [[Category: Wah, D A.]] | ||
| - | [[Category: MG]] | ||
[[Category: homodimer]] | [[Category: homodimer]] | ||
[[Category: peptide-binding pocket]] | [[Category: peptide-binding pocket]] | ||
[[Category: protein-peptide complex]] | [[Category: protein-peptide complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:50:27 2008'' |
Revision as of 19:50, 30 March 2008
| |||||||
| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | SSPB (Haemophilus influenzae) | ||||||
| Related: | 1OU9, 1OUL
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
structure of an AAA+ protease delivery protein in complex with a peptide degradation tag
Overview
Substrate selection by AAA+ ATPases that function to unfold proteins or alter protein conformation is often regulated by delivery or adaptor proteins. SspB is a protein dimer that binds to the ssrA degradation tag and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for degradation. Here, we describe the structure of the peptide binding domain of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution. The ssrA peptides are bound in well-defined clefts located at the extreme ends of the SspB homodimer. SspB contacts residues within the N-terminal and central regions of the 11 residue ssrA tag but leaves the C-terminal residues exposed and positioned to dock with ClpX. This structure, taken together with biochemical analysis of SspB, suggests mechanisms by which proteins like SspB escort substrates to AAA+ ATPases and enhance the specificity and affinity of target recognition.
About this Structure
1OU8 is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.
Reference
Structure of a delivery protein for an AAA+ protease in complex with a peptide degradation tag., Levchenko I, Grant RA, Wah DA, Sauer RT, Baker TA, Mol Cell. 2003 Aug;12(2):365-72. PMID:14536076
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