1ow4
From Proteopedia
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|PDB= 1ow4 |SIZE=350|CAPTION= <scene name='initialview01'>1ow4</scene>, resolution 1.60Å | |PDB= 1ow4 |SIZE=350|CAPTION= <scene name='initialview01'>1ow4</scene>, resolution 1.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=2AN:1-ANILINO-8-NAPHTHALENE+SULFONATE'>2AN</scene> | + | |LIGAND= <scene name='pdbligand=2AN:1-ANILINO-8-NAPHTHALENE+SULFONATE'>2AN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Pheromone Binding Protein (PBP) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6988 Leucophaea maderae]) | |GENE= Pheromone Binding Protein (PBP) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6988 Leucophaea maderae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1org|1ORG]], [[1p28|1P28]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow4 OCA], [http://www.ebi.ac.uk/pdbsum/1ow4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ow4 RCSB]</span> | ||
}} | }} | ||
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[[Category: Spinelli, S.]] | [[Category: Spinelli, S.]] | ||
[[Category: Tegoni, M.]] | [[Category: Tegoni, M.]] | ||
| - | [[Category: 2AN]] | ||
| - | [[Category: GOL]] | ||
[[Category: ligand 8-anilino-1-naphtalenesulfonic acid]] | [[Category: ligand 8-anilino-1-naphtalenesulfonic acid]] | ||
[[Category: pheromone binding protein]] | [[Category: pheromone binding protein]] | ||
[[Category: transport protein]] | [[Category: transport protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:51:13 2008'' |
Revision as of 19:51, 30 March 2008
| |||||||
| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | Pheromone Binding Protein (PBP) (Leucophaea maderae) | ||||||
| Related: | 1ORG, 1P28
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a pheromone binding protein from the cockroach Leucophaea maderae in complex with the fluorescent reporter ANS (1-anilinonaphtalene-8-sulfonic acid),
Overview
Pheromone-binding proteins (PBPs) are small helical proteins found in sensorial organs, particularly in the antennae, of moth and other insect species. They were proposed to solubilize and carry the hydrophobic pheromonal compounds through the antennal lymph to receptors, participating thus in the peri-receptor events of signal transduction. The x-ray structure of Bombyx mori PBP (BmorPBP), from male antennae, revealed a six-helix fold forming a cavity that contains the pheromone bombykol. We have identified a PBP (LmaPBP) from the cockroach Leucophaea maderae in the antennae of the females, the gender attracted by pheromones in this species. Here we report the crystal structure of LmaPBP alone or in complex with a fluorescent reporter (amino-naphthalen sulfonate, ANS) or with a component of the pheromonal blend, 3-hydroxy-butan-2-one. Both compounds bind in the internal cavity of LmaPBP, which is more hydrophilic than BmorPBP cavity. LmaPBP structure ends just after the sixth helix (helix F). BmorPBP structure extends beyond the sixth helix with a stretch of residues elongated at neutral pH and folding as a seventh internalized helix at low pH. These differences between LmaPBP and BmorPBP structures suggest that different binding and release mechanism may be adapted to the hydrophilicity or hydrophobicity of the pheromonal ligand.
About this Structure
1OW4 is a Single protein structure of sequence from Leucophaea maderae. Full crystallographic information is available from OCA.
Reference
The crystal structure of a cockroach pheromone-binding protein suggests a new ligand binding and release mechanism., Lartigue A, Gruez A, Spinelli S, Riviere S, Brossut R, Tegoni M, Cambillau C, J Biol Chem. 2003 Aug 8;278(32):30213-8. Epub 2003 May 23. PMID:12766173
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