This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Transposase
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
== Function == | == Function == | ||
| - | '''Transposases''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism. Transposase contains a DDE motif in its active site enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. <br /> | + | '''Transposases''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism<ref>PMID:19478801</ref>. Transposase contains a DDE motif in its active site enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. <br /> |
| - | *'''Mu transposase''' (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA.<br /> | + | *'''Mu transposase''' (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA<ref>PMID:7628012</ref>.<br /> |
| - | *'''Hermes transposase''' is a fly protein.<br /> | + | *'''Hermes transposase''' is a fly protein<ref>PMID:19450689</ref>.<br /> |
* '''Dra2 transposase''' is a ''Deinococcus radiodurans'' protein<br /> | * '''Dra2 transposase''' is a ''Deinococcus radiodurans'' protein<br /> | ||
| - | *'''Tn5 transposase''' active site contains the DDE motif which catalyzes the movement of the transposon<br /> | + | *'''Tn5 transposase''' active site contains the DDE motif which catalyzes the movement of the transposon<ref>PMID:18680433</ref>.<br /> |
| - | *'''Mos1 transposase''' mediates the movement of Mos1 transposon<br /> | + | *'''Mos1 transposase''' mediates the movement of Mos1 transposon<ref>PMID:10762422</ref><br /> |
| - | *'''Tc3 transposase''' mediates the movement of Tc3 transposon in ''C. elegans''<br /> | + | *'''Tc3 transposase''' mediates the movement of Tc3 transposon in ''C. elegans''<ref>PMID:7838706</ref><br /> |
== Disease == | == Disease == | ||
Revision as of 09:41, 21 September 2016
| |||||||||||
3D structures of transposase
Updated on 21-September-2016
References
- ↑ Ivics Z, Li MA, Mates L, Boeke JD, Nagy A, Bradley A, Izsvak Z. Transposon-mediated genome manipulation in vertebrates. Nat Methods. 2009 Jun;6(6):415-22. doi: 10.1038/nmeth.1332. PMID:19478801 doi:http://dx.doi.org/10.1038/nmeth.1332
- ↑ Rice P, Mizuuchi K. Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration. Cell. 1995 Jul 28;82(2):209-20. PMID:7628012
- ↑ Park JM, Evertts AG, Levin HL. The Hermes transposon of Musca domestica and its use as a mutagen of Schizosaccharomyces pombe. Methods. 2009 Nov;49(3):243-7. doi: 10.1016/j.ymeth.2009.05.004. Epub 2009 May, 18. PMID:19450689 doi:http://dx.doi.org/10.1016/j.ymeth.2009.05.004
- ↑ Reznikoff WS. Transposon Tn5. Annu Rev Genet. 2008;42:269-86. PMID:18680433 doi:http://dx.doi.org/10.1146/annurev.genet.42.110807.091656
- ↑ Wang W, Swevers L, Iatrou K. Mariner (Mos1) transposase and genomic integration of foreign gene sequences in Bombyx mori cells. Insect Mol Biol. 2000 Apr;9(2):145-55. PMID:10762422
- ↑ Colloms SD, van Luenen HG, Plasterk RH. DNA binding activities of the Caenorhabditis elegans Tc3 transposase. Nucleic Acids Res. 1994 Dec 25;22(25):5548-54. PMID:7838706
