1p1t
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= CSTF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= CSTF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p1t OCA], [http://www.ebi.ac.uk/pdbsum/1p1t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p1t RCSB]</span> | ||
}} | }} | ||
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[[Category: rna recognition motif]] | [[Category: rna recognition motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:53:36 2008'' |
Revision as of 19:53, 30 March 2008
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| Gene: | CSTF2 (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR Structure of the N-terminal RRM domain of Cleavage stimulation factor 64 KDa subunit
Overview
Vertebrate polyadenylation sites are identified by the AAUAAA signal and by GU-rich sequences downstream of the cleavage site. These are recognized by a heterotrimeric protein complex (CstF) through its 64 kDa subunit (CstF-64); the strength of this interaction affects the efficiency of poly(A) site utilization. We present the structure of the RNA-binding domain of CstF-64 containing an RNA recognition motif (RRM) augmented by N- and C-terminal helices. The C-terminal helix unfolds upon RNA binding and extends into the hinge domain where interactions with factors responsible for assembly of the polyadenylation complex occur. We propose that this conformational change initiates assembly. Consecutive Us are required for a strong CstF-GU interaction and we show how UU dinucleotides are recognized. Contacts outside the UU pocket fine tune the protein-RNA interaction and provide different affinities for distinct GU-rich elements. The protein-RNA interface remains mobile, most likely a requirement to bind many GU-rich sequences and yet discriminate against other RNAs. The structural distinction between sequences that form stable and unstable complexes provides an operational distinction between weakly and strongly processed poly(A) sites.
About this Structure
1P1T is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein., Perez Canadillas JM, Varani G, EMBO J. 2003 Jun 2;22(11):2821-30. PMID:12773396
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