1p4m
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1p4m |SIZE=350|CAPTION= <scene name='initialview01'>1p4m</scene>, resolution 1.80Å | |PDB= 1p4m |SIZE=350|CAPTION= <scene name='initialview01'>1p4m</scene>, resolution 1.80Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Riboflavin_kinase Riboflavin kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.26 2.7.1.26] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_kinase Riboflavin kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.26 2.7.1.26] </span> |
|GENE= FLJ11149 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= FLJ11149 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1nb0|1NB0]], [[1nb9|1NB9]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p4m OCA], [http://www.ebi.ac.uk/pdbsum/1p4m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p4m RCSB]</span> | ||
}} | }} | ||
| Line 29: | Line 32: | ||
[[Category: Zhang, H.]] | [[Category: Zhang, H.]] | ||
[[Category: Zhou, Q.]] | [[Category: Zhou, Q.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: FMN]] | ||
| - | [[Category: MG]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
[[Category: flavin mononucleotide]] | [[Category: flavin mononucleotide]] | ||
[[Category: riboflavin kinase]] | [[Category: riboflavin kinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:54:45 2008'' |
Revision as of 19:54, 30 March 2008
| |||||||
| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | FLJ11149 (Homo sapiens) | ||||||
| Activity: | Riboflavin kinase, with EC number 2.7.1.26 | ||||||
| Related: | 1NB0, 1NB9
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF RIBOFLAVIN KINASE
Overview
Riboflavin kinase (RFK) is an essential enzyme catalyzing the phosphorylation of riboflavin (vitamin B(2)) to form FMN, an obligatory step in vitamin B(2) utilization and flavin cofactor synthesis. The structure of human RFK revealed a six-stranded antiparallel beta barrel core structurally similar to the riboflavin synthase/ferredoxin reductase FAD binding domain fold. The binding site of an intrinsically bound MgADP defines a novel nucleotide binding motif that encompasses a loop, a 3(10) helix, and a reverse turn followed by a short beta strand. This active site loop forms an arch with ATP and riboflavin binding at the opposite side and the phosphoryl transfer appears to occur through the hole underneath the arch. The invariant residues Asn36 and Glu86 are implicated in the catalysis.
About this Structure
1P4M is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 1NBG. Full crystallographic information is available from OCA.
Reference
Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch., Karthikeyan S, Zhou Q, Mseeh F, Grishin NV, Osterman AL, Zhang H, Structure. 2003 Mar;11(3):265-73. PMID:12623014
Page seeded by OCA on Sun Mar 30 22:54:45 2008
