1p6t
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Copper-exporting_ATPase Copper-exporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.4 3.6.3.4] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Copper-exporting_ATPase Copper-exporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.4 3.6.3.4] </span> |
|GENE= YVGX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |GENE= YVGX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1oq3|1OQ3]], [[1kqk|1KQK]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p6t OCA], [http://www.ebi.ac.uk/pdbsum/1p6t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p6t RCSB]</span> | ||
}} | }} | ||
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[[Category: SPINE, Structural Proteomics in Europe.]] | [[Category: SPINE, Structural Proteomics in Europe.]] | ||
[[Category: Su, X C.]] | [[Category: Su, X C.]] | ||
| - | [[Category: | + | [[Category: beta-alpha-beta-beta-alpha-beta fold]] |
| + | [[Category: copa]] | ||
| + | [[Category: nmr]] | ||
| + | [[Category: p-type atpase]] | ||
[[Category: spine]] | [[Category: spine]] | ||
[[Category: structural genomic]] | [[Category: structural genomic]] | ||
[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
| + | [[Category: water-soluble region]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:55:42 2008'' |
Revision as of 19:55, 30 March 2008
| |||||||
| Gene: | YVGX (Bacillus subtilis) | ||||||
| Activity: | Copper-exporting ATPase, with EC number 3.6.3.4 | ||||||
| Related: | 1OQ3, 1KQK
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure characterization of the water soluble region of P-type ATPase CopA from Bacillus subtilis
Overview
The solution structure of the N-terminal region (151 amino acids) of a copper ATPase, CopA, from Bacillus subtilis, is reported here. It consists of two domains, CopAa and CopAb, linked by two amino acids. It is found that the two domains, which had already been separately characterized, interact one to the other through a hydrogen bond network and a few hydrophobic interactions, forming a single rigid body. The two metal binding sites are far from one another, and the short link between the domains prevents them from interacting. This and the surface electrostatic potential suggest that each domain receives copper from the copper chaperone, CopZ, independently and transfers it to the membrane binding site of CopA. The affinity constants of silver(I) and copper(I) are similar for the two sites as monitored by NMR. Because the present construct "domain-short link-domain" is shared also by the last two domains of the eukaryotic copper ATPases and several residues at the interface between the two domains are conserved, the conclusions of the present study have general validity for the understanding of the function of copper ATPases.
About this Structure
1P6T is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis., Banci L, Bertini I, Ciofi-Baffoni S, Gonnelli L, Su XC, J Biol Chem. 2003 Dec 12;278(50):50506-13. Epub 2003 Sep 27. PMID:14514665
Page seeded by OCA on Sun Mar 30 22:55:42 2008
Categories: Bacillus subtilis | Copper-exporting ATPase | Single protein | Banci, L. | Bertini, I. | Ciofi-Baffoni, S. | Gonnelli, L. | SPINE, Structural Proteomics in Europe. | Su, X C. | Beta-alpha-beta-beta-alpha-beta fold | Copa | Nmr | P-type atpase | Spine | Structural genomic | Structural proteomics in europe | Water-soluble region
