1p77
From Proteopedia
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|PDB= 1p77 |SIZE=350|CAPTION= <scene name='initialview01'>1p77</scene>, resolution 1.95Å | |PDB= 1p77 |SIZE=350|CAPTION= <scene name='initialview01'>1p77</scene>, resolution 1.95Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> | + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ATR:2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE'>ATR</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span> |
|GENE= AROE OR HI0655 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae]) | |GENE= AROE OR HI0655 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1p74|1P74]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p77 OCA], [http://www.ebi.ac.uk/pdbsum/1p77 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p77 RCSB]</span> | ||
}} | }} | ||
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[[Category: Swanson, R V.]] | [[Category: Swanson, R V.]] | ||
[[Category: Ye, S.]] | [[Category: Ye, S.]] | ||
| - | [[Category: ACT]] | ||
| - | [[Category: ATR]] | ||
[[Category: haemophilus influenzae]] | [[Category: haemophilus influenzae]] | ||
[[Category: nadph]] | [[Category: nadph]] | ||
[[Category: shikimate dehydrogenase]] | [[Category: shikimate dehydrogenase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:55:52 2008'' |
Revision as of 19:55, 30 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | AROE OR HI0655 (Haemophilus influenzae) | ||||||
| Activity: | Shikimate dehydrogenase, with EC number 1.1.1.25 | ||||||
| Related: | 1P74
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF SHIKIMATE DEHYDROGENASE (AROE) FROM HAEMOPHILUS INFLUENZAE
Overview
Shikimate dehydrogenase catalyzes the NADPH-dependent reversible reduction of 3-dehydroshikimate to shikimate. We report the first X-ray structure of shikimate dehydrogenase from Haemophilus influenzae to 2.4-A resolution and its complex with NADPH to 1.95-A resolution. The molecule contains two domains, a catalytic domain with a novel open twisted alpha/beta motif and an NADPH binding domain with a typical Rossmann fold. The enzyme contains a unique glycine-rich P-loop with a conserved sequence motif, GAGGXX, that results in NADPH adopting a nonstandard binding mode with the nicotinamide and ribose moieties disordered in the binary complex. A deep pocket with a narrow entrance between the two domains, containing strictly conserved residues primarily contributed by the catalytic domain, is identified as a potential 3-dehydroshikimate binding pocket. The flexibility of the nicotinamide mononucleotide portion of NADPH may be necessary for the substrate 3-dehydroshikimate to enter the pocket and for the release of the product shikimate.
About this Structure
1P77 is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.
Reference
The crystal structure of shikimate dehydrogenase (AroE) reveals a unique NADPH binding mode., Ye S, Von Delft F, Brooun A, Knuth MW, Swanson RV, McRee DE, J Bacteriol. 2003 Jul;185(14):4144-51. PMID:12837789
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