This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


User:Wally Novak/Sandbox Brown

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
== Introduction ==
== Introduction ==
<StructureSection load='4qyz' size='340' side='right' caption='CRISPR RNA-guided surveillance complex with target ssDNA' scene=''>
<StructureSection load='4qyz' size='340' side='right' caption='CRISPR RNA-guided surveillance complex with target ssDNA' scene=''>
-
Cas9 is a large multifunctional protein that plays a central role in the CRISPR-Cas adaptive defense mechanism found in a vast amount of bacteria and archaea <ref>DOI 10.1126/science.1258096</ref>.
+
Cas9 is a large multifunctional protein that plays a central role in the CRISPR-Cas adaptive defense mechanism found in a vast amount of bacteria and archaea <ref>DOI 10.1126/science.1258096</ref>. It accomplishes this through the use of antisense RNAs which serve as signatures from past viral invasions <ref>DOI 10.1186/1745-6150-1-7</ref>. The adaptive immunity occurs in three stages: insertion of invading DNA into CRISPR locus, transcription of precursor crRNA from CRISPR locus that will be used to generate crRNA, and crRNA-directed cleavage of foreign nucleic acids by cas9 <ref>DOI 10.1126/science.1258096</ref>.
This is a default text for your page '''Wally Novak/Sandbox Brown'''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.
This is a default text for your page '''Wally Novak/Sandbox Brown'''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.

Revision as of 23:19, 10 October 2016

Introduction

CRISPR RNA-guided surveillance complex with target ssDNA

Drag the structure with the mouse to rotate

References

  1. ↑ Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014 Nov 28;346(6213):1258096. doi: 10.1126/science.1258096. PMID:25430774 doi:http://dx.doi.org/10.1126/science.1258096
  2. ↑ Makarova KS, Grishin NV, Shabalina SA, Wolf YI, Koonin EV. A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct. 2006 Mar 16;1:7. PMID:16545108 doi:http://dx.doi.org/10.1186/1745-6150-1-7
  3. ↑ Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014 Nov 28;346(6213):1258096. doi: 10.1126/science.1258096. PMID:25430774 doi:http://dx.doi.org/10.1126/science.1258096
  4. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
  5. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644

Proteopedia Page Contributors and Editors (what is this?)

Wally Novak

Retrieved from "http://52.214.119.220/wiki/index.php/User:Wally_Novak/Sandbox_Brown"
Personal tools