User:Wally Novak/Sandbox Brown
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | == | + | == Natural Role == |
<StructureSection load='4qyz' size='340' side='right' caption='CRISPR RNA-guided surveillance complex with target ssDNA' scene=''> | <StructureSection load='4qyz' size='340' side='right' caption='CRISPR RNA-guided surveillance complex with target ssDNA' scene=''> | ||
| - | Cas9 is a large multifunctional protein that plays a central role in the CRISPR-Cas adaptive defense mechanism found in a vast amount of bacteria and archaea <ref name='one'>DOI 10.1126/science.1258096</ref>. It accomplishes this through the use of antisense RNAs which serve as signatures from past viral invasions <ref>DOI 10.1186/1745-6150-1-7</ref>. The adaptive immunity occurs in three stages: insertion of invading DNA into CRISPR locus, transcription of precursor crRNA from CRISPR locus that will be used to generate crRNA, and crRNA-directed cleavage of foreign nucleic acids by cas9 <ref name='one'/>. | + | Cas9 is a large multifunctional protein that plays a central role in the CRISPR-Cas adaptive defense mechanism found in a vast amount of bacteria and archaea <ref name='one'>DOI 10.1126/science.1258096</ref>. It accomplishes this through the use of antisense RNAs which serve as signatures from past viral invasions <ref>DOI 10.1186/1745-6150-1-7</ref>. The adaptive immunity occurs in three stages: insertion of invading DNA into CRISPR locus, transcription of precursor crRNA from CRISPR locus that will be used to generate crRNA, and crRNA-directed cleavage of foreign nucleic acids by cas9. PAM (protospacer adjacent motif) sequences must be present adjacent to the crRNA-targeted sequence to be cleaved <ref name='one'/>. Just in the last few years, this defensive mechanism and the cas9 protein has been used to develop genome engineering applications. |
This is a default text for your page '''Wally Novak/Sandbox Brown'''. Click above on '''edit this page''' to modify. Be careful with the < and > signs. | This is a default text for your page '''Wally Novak/Sandbox Brown'''. Click above on '''edit this page''' to modify. Be careful with the < and > signs. | ||
| Line 7: | Line 7: | ||
== Role in CRISPR == | == Role in CRISPR == | ||
| + | |||
| + | [[Image:cas9image.jpg]] | ||
== Overall Structure == | == Overall Structure == | ||
| + | The Cas9 protein contains two nuclease domains, HNH and RuvC | ||
== Active Site Structure == | == Active Site Structure == | ||
Revision as of 23:40, 10 October 2016
Natural Role
| |||||||||||
References
- ↑ 1.0 1.1 Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014 Nov 28;346(6213):1258096. doi: 10.1126/science.1258096. PMID:25430774 doi:http://dx.doi.org/10.1126/science.1258096
- ↑ Makarova KS, Grishin NV, Shabalina SA, Wolf YI, Koonin EV. A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct. 2006 Mar 16;1:7. PMID:16545108 doi:http://dx.doi.org/10.1186/1745-6150-1-7
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
