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| - | ==Solution NMR structure of Tetrahymena telomerase RNA pseudoknot==
| + | #REDIRECT [[5kmz]] This PDB entry is obsolete and replaced by 5kmz |
| - | <StructureSection load='2n6q' size='340' side='right' caption='[[2n6q]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[2n6q]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N6Q FirstGlance]. <br>
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| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6q OCA], [http://pdbe.org/2n6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n6q RCSB], [http://www.ebi.ac.uk/pdbsum/2n6q PDBsum]</span></td></tr>
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| - | </table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-ends, using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). We report the cryo-electron microscopy structure of Tetrahymena telomerase at ~9 angstrom resolution. In addition to seven known holoenzyme proteins, we identify two additional proteins that form a complex (TEB) with single-stranded telomere DNA-binding protein Teb1, paralogous to heterotrimeric replication protein A (RPA). The p75-p45-p19 subcomplex is identified as another RPA-related complex, CST (CTC1-STN1-TEN1). This study reveals the paths of TER in the TERT-TER-p65 catalytic core and single-stranded DNA exit; extensive subunit interactions of the TERT essential N-terminal domain, p50, and TEB; and other subunit identities and structures, including p19 and p45C crystal structures. Our findings provide structural and mechanistic insights into telomerase holoenzyme function.
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| - | Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions.,Jiang J, Chan H, Cash DD, Miracco EJ, Ogorzalek Loo RR, Upton HE, Cascio D, O'Brien Johnson R, Collins K, Loo JA, Zhou ZH, Feigon J Science. 2015 Oct 30;350(6260):aab4070. doi: 10.1126/science.aab4070. Epub 2015, Oct 15. PMID:26472759<ref>PMID:26472759</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 2n6q" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Cash, D D]]
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| - | [[Category: Feigon, J]]
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| - | [[Category: Pseudoknot]]
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| - | [[Category: Rna]]
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| - | [[Category: Telomerase]]
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| - | [[Category: Triplex]]
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