5gqo
From Proteopedia
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- | '''Unreleased structure''' | ||
- | + | ==Structure of the second Single Stranded DNA Binding protein (SSBb) from Mycobacterium smegmatis== | |
+ | <StructureSection load='5gqo' size='340' side='right' caption='[[5gqo]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[5gqo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GQO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GQO FirstGlance]. <br> | ||
+ | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gqo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gqo OCA], [http://pdbe.org/5gqo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gqo RCSB], [http://www.ebi.ac.uk/pdbsum/5gqo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gqo ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | All mycobacteria with sequenced genomes, except M. leprae, have a second Single Stranded DNA Binding protein (SSBb) in addition to the canonical one (SSBa). This paralogue from M. smegmatis (MsSSBb) has been cloned, expressed and purified. The protein, which is probably involved in stress response, has been crystallized and X-ray analyzed in the first structure elucidation of a mycobacterial SSBb. In spite of the low sequence identity between SSBas and SSBbs in mycobacteria, the tertiary and quaternary structure of the DNA binding domain of MsSSBb is similar to that observed in mycobacterial SSBas. In particular, the quaternary structure is 'clamped' using a C-terminal stretch of the N-domain, which endows the tetrameric molecule with additional stability and its characteristic shape. Comparison involving available, rather limited, structural data on SSBbs from other sources, appears to suggest that SSBbs could exhibit higher structural variability than SSBas do. | ||
- | + | Structure of the second Single Stranded DNA Binding protein (SSBb) from Mycobacterium smegmatis.,Singh A, Varshney U, Vijayan M J Struct Biol. 2016 Sep 19. pii: S1047-8477(16)30201-5. doi:, 10.1016/j.jsb.2016.09.012. PMID:27659385<ref>PMID:27659385</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
+ | <div class="pdbe-citations 5gqo" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </StructureSection> | ||
+ | [[Category: Singh, A]] | ||
+ | [[Category: Varshney, U]] | ||
+ | [[Category: Vijayan, M]] | ||
+ | [[Category: Dna binding protein]] | ||
+ | [[Category: Inter-subunit clamp]] | ||
+ | [[Category: Ob fold]] | ||
+ | [[Category: Paralogous ssb]] | ||
+ | [[Category: Quaternary structure]] | ||
+ | [[Category: Variability]] |
Revision as of 17:38, 19 October 2016
Structure of the second Single Stranded DNA Binding protein (SSBb) from Mycobacterium smegmatis
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