5b6l
From Proteopedia
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/HHOA_SYNY3 HHOA_SYNY3]] A putative protease, its function overlaps that of the related putative proteases HhoB and HtrA.<ref>PMID:16912048</ref> | [[http://www.uniprot.org/uniprot/HHOA_SYNY3 HHOA_SYNY3]] A putative protease, its function overlaps that of the related putative proteases HhoB and HtrA.<ref>PMID:16912048</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The high temperature requirement A (HtrA) proteases are oligomeric serine proteases essential for protein quality control. HtrA homolog A (HhoA) from the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 assembles into a proteolytically active hexamer. Herein, we present the crystal structure of the hexameric HhoA in complex with the copurified peptide. Our data indicate the presence of three methionines in close proximity to the peptide-binding site of the PDZ domain. Unexpectedly, we observed that a zinc ion is accommodated within the central channel formed by a HhoA trimer. However, neither calcium nor magnesium showed affinity for HhoA. The role of the zinc ion in HhoA was tested in an in vitro proteolytic assay against the nonspecific substrate beta-casein and was found to be inhibitory. Our findings provide insights into the regulation of HhoA by a redox-related mechanism involving methionine residues and by zinc ion-binding within the central channel. | ||
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| + | Crystal structure of the zinc-bound HhoA protease from Synechocystis sp. PCC 6803.,Dong W, Wang J, Niu G, Zhao S, Liu L FEBS Lett. 2016 Oct;590(19):3435-3442. doi: 10.1002/1873-3468.12416. Epub 2016, Sep 23. PMID:27616292<ref>PMID:27616292</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5b6l" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 11:25, 26 October 2016
Structure of Deg protease HhoA from Synechocystis sp. PCC 6803
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Categories: Dong, W | Liu, L | Wang, J | Hydrolase | Serine protease
