3jaz
From Proteopedia
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==Atomic model of cytoplasmic polyhedrosis virus with ATP== | ==Atomic model of cytoplasmic polyhedrosis virus with ATP== | ||
<StructureSection load='3jaz' size='340' side='right' caption='[[3jaz]], [[Resolution|resolution]] 3.10Å' scene=''> | <StructureSection load='3jaz' size='340' side='right' caption='[[3jaz]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
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<table><tr><td colspan='2'>[[3jaz]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAZ FirstGlance]. <br> | <table><tr><td colspan='2'>[[3jaz]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAZ FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3jay|3jay]], [[3jb0|3jb0]], [[3jb1|3jb1]], [[3jb2|3jb2]], [[3jb3|3jb3]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3jay|3jay]], [[3jb0|3jb0]], [[3jb1|3jb1]], [[3jb2|3jb2]], [[3jb3|3jb3]]</td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jaz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jaz RCSB], [http://www.ebi.ac.uk/pdbsum/3jaz PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jaz OCA], [http://pdbe.org/3jaz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jaz RCSB], [http://www.ebi.ac.uk/pdbsum/3jaz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jaz ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/CAPSD_CPVBM CAPSD_CPVBM]] Capsid protein self-assembles to form an icosahedral capsid with a pseudo T=2 symmetry, about 50 nm in diameter, and consisting of 120 capsid proteins. The capsid encapsulates the genomic RNA. | [[http://www.uniprot.org/uniprot/CAPSD_CPVBM CAPSD_CPVBM]] Capsid protein self-assembles to form an icosahedral capsid with a pseudo T=2 symmetry, about 50 nm in diameter, and consisting of 120 capsid proteins. The capsid encapsulates the genomic RNA. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | mRNA transcription in dsRNA viruses is a highly regulated process but the mechanism of this regulation is not known. Here, by nucleoside triphosphatase (NTPase) assay and comparisons of six high-resolution (2.9-3.1 A) cryo-electron microscopy structures of cytoplasmic polyhedrosis virus with bound ligands, we show that the large sub-domain of the guanylyltransferase (GTase) domain of the turret protein (TP) also has an ATP-binding site and is likely an ATPase. S-adenosyl-L-methionine (SAM) acts as a signal and binds the methylase-2 domain of TP to induce conformational change of the viral capsid, which in turn activates the putative ATPase. ATP binding/hydrolysis leads to an enlarged capsid for efficient mRNA synthesis, an open GTase domain for His217-mediated guanylyl transfer, and an open methylase-1 domain for SAM binding and methyl transfer. Taken together, our data support a role of the putative ATPase in mediating the activation of mRNA transcription and capping within the confines of the virus. | ||
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| + | A putative ATPase mediates RNA transcription and capping in a dsRNA virus.,Yu X, Jiang J, Sun J, Zhou ZH Elife. 2015 Aug 4;4:e07901. doi: 10.7554/eLife.07901. PMID:26240998<ref>PMID:26240998</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3jaz" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 11:09, 2 November 2016
Atomic model of cytoplasmic polyhedrosis virus with ATP
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