1pt1
From Proteopedia
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|PDB= 1pt1 |SIZE=350|CAPTION= <scene name='initialview01'>1pt1</scene>, resolution 1.90Å | |PDB= 1pt1 |SIZE=350|CAPTION= <scene name='initialview01'>1pt1</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Aspartate_1-decarboxylase Aspartate 1-decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.11 4.1.1.11] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_1-decarboxylase Aspartate 1-decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.11 4.1.1.11] </span> |
|GENE= PAND ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= PAND ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1aw8|1AW8]], [[1ppy|1PPY]], [[1pqe|1PQE]], [[1pqf|1PQF]], [[1pqh|1PQH]], [[1pt0|1PT0]], [[1pyq|1PYQ]], [[1pyu|1PYU]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pt1 OCA], [http://www.ebi.ac.uk/pdbsum/1pt1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pt1 RCSB]</span> | ||
}} | }} | ||
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[[Category: Webb, M E.]] | [[Category: Webb, M E.]] | ||
[[Category: Witty, M.]] | [[Category: Witty, M.]] | ||
| - | [[Category: SO4]] | ||
[[Category: decarboxylase]] | [[Category: decarboxylase]] | ||
[[Category: pantothenate pathway]] | [[Category: pantothenate pathway]] | ||
[[Category: protein self-processing]] | [[Category: protein self-processing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:04:09 2008'' |
Revision as of 20:04, 30 March 2008
| |||||||
| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | PAND (Escherichia coli) | ||||||
| Activity: | Aspartate 1-decarboxylase, with EC number 4.1.1.11 | ||||||
| Related: | 1AW8, 1PPY, 1PQE, 1PQF, 1PQH, 1PT0, 1PYQ, 1PYU
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Unprocessed Pyruvoyl Dependent Aspartate Decarboxylase with Histidine 11 Mutated to Alanine
Overview
Aspartate decarboxylase, which is translated as a pro-protein, undergoes intramolecular self-cleavage at Gly24-Ser25. We have determined the crystal structures of an unprocessed native precursor, in addition to Ala24 insertion, Ala26 insertion and Gly24-->Ser, His11-->Ala, Ser25-->Ala, Ser25-->Cys and Ser25-->Thr mutants. Comparative analyses of the cleavage site reveal specific conformational constraints that govern self-processing and demonstrate that considerable rearrangement must occur. We suggest that Thr57 Ogamma and a water molecule form an 'oxyanion hole' that likely stabilizes the proposed oxyoxazolidine intermediate. Thr57 and this water molecule are probable catalytic residues able to support acid-base catalysis. The conformational freedom in the loop preceding the cleavage site appears to play a determining role in the reaction. The molecular mechanism of self-processing, presented here, emphasizes the importance of stabilization of the oxyoxazolidine intermediate. Comparison of the structural features shows significant similarity to those in other self-processing systems, and suggests that models of the cleavage site of such enzymes based on Ser-->Ala or Ser-->Thr mutants alone may lead to erroneous interpretations of the mechanism.
About this Structure
1PT1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural constraints on protein self-processing in L-aspartate-alpha-decarboxylase., Schmitzberger F, Kilkenny ML, Lobley CM, Webb ME, Vinkovic M, Matak-Vinkovic D, Witty M, Chirgadze DY, Smith AG, Abell C, Blundell TL, EMBO J. 2003 Dec 1;22(23):6193-204. PMID:14633979
Page seeded by OCA on Sun Mar 30 23:04:09 2008
Categories: Aspartate 1-decarboxylase | Escherichia coli | Single protein | Abell, C. | Blundell, T L. | Chirgadze, D Y. | Kilkenny, M L. | Lobley, C M.C. | Matak-Vinkovic, D. | Schmitzberger, F. | Smith, A G. | Vinkovic, M. | Webb, M E. | Witty, M. | Decarboxylase | Pantothenate pathway | Protein self-processing
