5gki

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m (Protected "5gki" [edit=sysop:move=sysop])
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'''Unreleased structure'''
 
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The entry 5gki is ON HOLD
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==Structure of EndoMS-dsDNA3 complex==
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<StructureSection load='5gki' size='340' side='right' caption='[[5gki]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5gki]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GKI FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5gke|5gke]], [[5gkf|5gkf]], [[5gkg|5gkg]], [[5gkh|5gkh]], [[5gkj|5gkj]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gki OCA], [http://pdbe.org/5gki PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gki RCSB], [http://www.ebi.ac.uk/pdbsum/5gki PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gki ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/NUCS_THEKO NUCS_THEKO]] Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Archaeal NucS nuclease was thought to degrade the single-stranded region of branched DNA, which contains flapped and splayed DNA. However, recent findings indicated that EndoMS, the orthologous enzyme of NucS, specifically cleaves double-stranded DNA (dsDNA) containing mismatched bases. In this study, we determined the structure of the EndoMS-DNA complex. The complex structure of the EndoMS dimer with dsDNA unexpectedly revealed that the mismatched bases were flipped out into binding sites, and the overall architecture most resembled that of restriction enzymes. The structure of the apo form was similar to the reported structure of Pyrococcus abyssi NucS, indicating that movement of the C-terminal domain from the resting state was required for activity. In addition, a model of the EndoMS-PCNA-DNA complex was preliminarily verified with electron microscopy. The structures strongly support the idea that EndoMS acts in a mismatch repair pathway.
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Authors:
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Structure of the EndoMS-DNA Complex as Mismatch Restriction Endonuclease.,Nakae S, Hijikata A, Tsuji T, Yonezawa K, Kouyama KI, Mayanagi K, Ishino S, Ishino Y, Shirai T Structure. 2016 Oct 17. pii: S0969-2126(16)30294-5. doi:, 10.1016/j.str.2016.09.005. PMID:27773688<ref>PMID:27773688</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 5gki" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Hijikata, A]]
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[[Category: Ishino, S]]
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[[Category: Ishino, Y]]
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[[Category: Kouyama, K]]
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[[Category: Mayanagi, K]]
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[[Category: Nakae, S]]
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[[Category: Shirai, T]]
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[[Category: Tsuji, T]]
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[[Category: Yonezawa, K]]
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[[Category: Dna complex]]
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[[Category: Endonuclease]]
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[[Category: Hydrolase-dna complex]]

Revision as of 18:20, 2 November 2016

Structure of EndoMS-dsDNA3 complex

5gki, resolution 2.90Å

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