5gke

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m (Protected "5gke" [edit=sysop:move=sysop])
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'''Unreleased structure'''
 
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The entry 5gke is ON HOLD
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==Structure of EndoMS-dsDNA1 complex==
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<StructureSection load='5gke' size='340' side='right' caption='[[5gke]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5gke]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GKE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GKE FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5gkf|5gkf]], [[5gkg|5gkg]], [[5gkh|5gkh]], [[5gki|5gki]], [[5gkj|5gkj]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gke OCA], [http://pdbe.org/5gke PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gke RCSB], [http://www.ebi.ac.uk/pdbsum/5gke PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gke ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/NUCS_THEKO NUCS_THEKO]] Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Archaeal NucS nuclease was thought to degrade the single-stranded region of branched DNA, which contains flapped and splayed DNA. However, recent findings indicated that EndoMS, the orthologous enzyme of NucS, specifically cleaves double-stranded DNA (dsDNA) containing mismatched bases. In this study, we determined the structure of the EndoMS-DNA complex. The complex structure of the EndoMS dimer with dsDNA unexpectedly revealed that the mismatched bases were flipped out into binding sites, and the overall architecture most resembled that of restriction enzymes. The structure of the apo form was similar to the reported structure of Pyrococcus abyssi NucS, indicating that movement of the C-terminal domain from the resting state was required for activity. In addition, a model of the EndoMS-PCNA-DNA complex was preliminarily verified with electron microscopy. The structures strongly support the idea that EndoMS acts in a mismatch repair pathway.
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Authors:
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Structure of the EndoMS-DNA Complex as Mismatch Restriction Endonuclease.,Nakae S, Hijikata A, Tsuji T, Yonezawa K, Kouyama KI, Mayanagi K, Ishino S, Ishino Y, Shirai T Structure. 2016 Oct 17. pii: S0969-2126(16)30294-5. doi:, 10.1016/j.str.2016.09.005. PMID:27773688<ref>PMID:27773688</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 5gke" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Hijikata, A]]
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[[Category: Ishino, S]]
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[[Category: Ishino, Y]]
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[[Category: Kouyama, K]]
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[[Category: Mayanagi, K]]
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[[Category: Nakae, S]]
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[[Category: Shirai, T]]
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[[Category: Tsuji, T]]
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[[Category: Yonezawa, K]]
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[[Category: Dna-binding]]
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[[Category: Endonuclease]]
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[[Category: Hydrolase-dna complex]]

Revision as of 18:21, 2 November 2016

Structure of EndoMS-dsDNA1 complex

5gke, resolution 2.40Å

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