1pzb

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|PDB= 1pzb |SIZE=350|CAPTION= <scene name='initialview01'>1pzb</scene>, resolution 1.8&Aring;
|PDB= 1pzb |SIZE=350|CAPTION= <scene name='initialview01'>1pzb</scene>, resolution 1.8&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene>
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|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pzb OCA], [http://www.ebi.ac.uk/pdbsum/1pzb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pzb RCSB]</span>
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Petratos, K.]]
[[Category: Petratos, K.]]
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[[Category: CU]]
 
[[Category: electron transfer(cuproprotein)]]
[[Category: electron transfer(cuproprotein)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:30:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:06:37 2008''

Revision as of 20:06, 30 March 2008


PDB ID 1pzb

Drag the structure with the mouse to rotate
, resolution 1.8Å
Ligands:
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



THE CRYSTAL STRUCTURES OF REDUCED PSEUDOAZURIN FROM ALCALIGENES FAECALIS S-6 AT TWO PH VALUES


Overview

The structures of the reduced (Cu1+) blue-copper protein pseudoazurin from Alcaligenes faecalis strain S-6 are refined at pH 7.8 and 4.4 using X-ray diffraction data to 1.8 A resolution. The final R-factors for the high and low pH structures are 0.178 and 0.177, respectively. Comparing the reduced pseudoazurin at pH 7.8 with the oxidised (Cu2+) molecule, small changes are observed in the vicinity of the copper site and on the protein surface. At pH 4.4 the copper substituent imidazole of His81 rotates away from the metal with a concurrent movement of the latter towards the plane of the remaining three ligands (S gamma-Cys78, N delta 1-His40 and S delta-Met86) thus the geometry of the copper site becomes planar trigonal.

About this Structure

1PZB is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.

Reference

The crystal structures of reduced pseudoazurin from Alcaligenes faecalis S-6 at two pH values., Vakoufari E, Wilson KS, Petratos K, FEBS Lett. 1994 Jun 27;347(2-3):203-6. PMID:8034003

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