1q0b
From Proteopedia
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|PDB= 1q0b |SIZE=350|CAPTION= <scene name='initialview01'>1q0b</scene>, resolution 1.9Å | |PDB= 1q0b |SIZE=350|CAPTION= <scene name='initialview01'>1q0b</scene>, resolution 1.9Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAT:ETHYL+4-(3-HYDROXYPHENYL)-6-METHYL-2-THIOXO-1,2,3,4-TETRAHYDROPYRIMIDINE-5-CARBOXYLATE'>NAT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= KIF11 OR KNSL1 OR EG5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= KIF11 OR KNSL1 OR EG5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q0b OCA], [http://www.ebi.ac.uk/pdbsum/1q0b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q0b RCSB]</span> | ||
}} | }} | ||
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[[Category: Xu, B.]] | [[Category: Xu, B.]] | ||
[[Category: Yan, Y.]] | [[Category: Yan, Y.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: NAT]] | ||
[[Category: cell cycle]] | [[Category: cell cycle]] | ||
[[Category: monastrol]] | [[Category: monastrol]] | ||
[[Category: motor protein]] | [[Category: motor protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:07:05 2008'' |
Revision as of 20:07, 30 March 2008
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| , resolution 1.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | KIF11 OR KNSL1 OR EG5 (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the motor protein KSP in complex with ADP and monastrol
Overview
We report here the first inhibitor-bound structure of a mitotic motor protein. The 1.9 A resolution structure of the motor domain of KSP, bound with the small molecule monastrol and Mg2+ x ADP, reveals that monastrol confers inhibition by "induced-fitting" onto the protein some 12 A away from the catalytic center of the enzyme, resulting in the creation of a previously non-existing binding pocket. The structure provides new insights into the biochemical and mechanical mechanisms of the mitotic motor domain. Inhibition of KSP provides a novel mechanism to arrest mitotic spindle formation, a target of several approved and investigative anti-cancer agents. The structural information gleaned from this novel pocket offers a new angle for the design of anti-mitotic agents.
About this Structure
1Q0B is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Inhibition of a mitotic motor protein: where, how, and conformational consequences., Yan Y, Sardana V, Xu B, Homnick C, Halczenko W, Buser CA, Schaber M, Hartman GD, Huber HE, Kuo LC, J Mol Biol. 2004 Jan 9;335(2):547-54. PMID:14672662
Page seeded by OCA on Sun Mar 30 23:07:05 2008
Categories: Homo sapiens | Single protein | Buser, C A. | Halczenko, W. | Hartman, G D. | Homnick, C. | Huber, H E. | Kuo, L C. | Sardana, V. | Xu, B. | Yan, Y. | Cell cycle | Monastrol | Motor protein
