UDP-galactose 4-epimerase
From Proteopedia
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| - | <StructureSection load='2cnb' size='350' side='right' caption='Structure of UDP-galactose 4 epimerase complex with NAD | + | <StructureSection load='2cnb' size='350' side='right' caption='Structure of UDP-galactose 4 epimerase complex with NAD and UDP-galactose (PDB entry [[2cnb]])' scene=''> |
== Function == | == Function == | ||
'''UDP-galactose 4-epimerase''' (GalE) reversibly converts UDP-galactose to UDP-glucose. GalE catalyzes the last step in galactose metabolism<ref>PMID:12534808</ref>. GalE uses NAD+ as a cofactor. | '''UDP-galactose 4-epimerase''' (GalE) reversibly converts UDP-galactose to UDP-glucose. GalE catalyzes the last step in galactose metabolism<ref>PMID:12534808</ref>. GalE uses NAD+ as a cofactor. | ||
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== Disease == | == Disease == | ||
Mutations in GalE are the cause of galactosemia which is autosomal recessive disorder<ref>PMID:16301867</ref>. | Mutations in GalE are the cause of galactosemia which is autosomal recessive disorder<ref>PMID:16301867</ref>. | ||
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| + | == Structural highlights == | ||
| + | GalE active site contains the substrate UDP-galactose (UDP-gal) and the cofactor NAD. Tyrosine residue is the catalytic base which produces the ketose intermediate<ref>PMID:16946458</ref>. | ||
</StructureSection> | </StructureSection> | ||
Revision as of 08:26, 5 December 2016
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3D structures of UDP-galactose 4 epimerase
Updated on 05-December-2016
References
- ↑ Mozzi F, Savoy de Giori G, Font de Valdez G. UDP-galactose 4-epimerase: a key enzyme in exopolysaccharide formation by Lactobacillus casei CRL 87 in controlled pH batch cultures. J Appl Microbiol. 2003;94(2):175-83. PMID:12534808
- ↑ Park HD, Park KU, Kim JQ, Shin CH, Yang SW, Lee DH, Song YH, Song J. The molecular basis of UDP-galactose-4-epimerase (GALE) deficiency galactosemia in Korean patients. Genet Med. 2005 Nov-Dec;7(9):646-9. PMID:16301867 doi:10.109701.gim.0000194023.27802.2d
- ↑ Alphey MS, Burton A, Urbaniak MD, Boons GJ, Ferguson MA, Hunter WN. Trypanosoma brucei UDP-galactose-4'-epimerase in ternary complex with NAD+ and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):829-34. Epub 2006 Aug 11. PMID:16946458 doi:10.1107/S1744309106028740
