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1q4q
From Proteopedia
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|PDB= 1q4q |SIZE=350|CAPTION= <scene name='initialview01'>1q4q</scene>, resolution 2.1Å | |PDB= 1q4q |SIZE=350|CAPTION= <scene name='initialview01'>1q4q</scene>, resolution 2.1Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1jd4|1JD4]], [[1jd5|1JD5]], [[1jd6|1JD6]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q4q OCA], [http://www.ebi.ac.uk/pdbsum/1q4q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q4q RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Shi, Y.]] | [[Category: Shi, Y.]] | ||
[[Category: Yan, N.]] | [[Category: Yan, N.]] | ||
| - | [[Category: ZN]] | ||
[[Category: apoptosis]] | [[Category: apoptosis]] | ||
[[Category: caspase]] | [[Category: caspase]] | ||
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[[Category: ubiquitination]] | [[Category: ubiquitination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:08:48 2008'' |
Revision as of 20:08, 30 March 2008
| |||||||
| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Related: | 1JD4, 1JD5, 1JD6
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a DIAP1-Dronc complex
Overview
The inhibitor of apoptosis protein DIAP1 inhibits Dronc-dependent cell death by ubiquitinating Dronc. The pro-death proteins Reaper, Hid and Grim (RHG) promote apoptosis by antagonizing DIAP1 function. Here we report the structural basis of Dronc recognition by DIAP1 as well as a novel mechanism by which the RHG proteins remove DIAP1-mediated downregulation of Dronc. Biochemical and structural analyses revealed that the second BIR (BIR2) domain of DIAP1 recognizes a 12-residue sequence in Dronc. This recognition is essential for DIAP1 binding to Dronc, and for targeting Dronc for ubiquitination. Notably, the Dronc-binding surface on BIR2 coincides with that required for binding to the N termini of the RHG proteins, which competitively eliminate DIAP1-mediated ubiquitination of Dronc. These observations reveal the molecular mechanisms of how DIAP1 recognizes Dronc, and more importantly, how the RHG proteins remove DIAP1-mediated ubiquitination of Dronc.
About this Structure
1Q4Q is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Molecular mechanism of Reaper-Grim-Hid-mediated suppression of DIAP1-dependent Dronc ubiquitination., Chai J, Yan N, Huh JR, Wu JW, Li W, Hay BA, Shi Y, Nat Struct Biol. 2003 Nov;10(11):892-8. Epub 2003 Sep 28. PMID:14517550
Page seeded by OCA on Sun Mar 30 23:08:48 2008
Categories: Drosophila melanogaster | Protein complex | Chai, J. | Shi, Y. | Yan, N. | Apoptosis | Caspase | Iap | Mechanism | Ubiquitination
