5lk4
From Proteopedia
(Difference between revisions)
m (Protected "5lk4" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the Red Fluorescent Protein mScarlet at pH 7.8== | |
| + | <StructureSection load='5lk4' size='340' side='right' caption='[[5lk4]], [[Resolution|resolution]] 1.47Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5lk4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LK4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lk4 OCA], [http://pdbe.org/5lk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lk4 RCSB], [http://www.ebi.ac.uk/pdbsum/5lk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lk4 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red Forster proteins as a fusion tag, and it is especially useful as a fluorescence resonance energy transfer (FRET) acceptor in ratiometric imaging. | ||
| - | + | mScarlet: a bright monomeric red fluorescent protein for cellular imaging.,Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. PMID:27869816<ref>PMID:27869816</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5lk4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aumonier, S]] | ||
| + | [[Category: Gotthard, G]] | ||
| + | [[Category: Royant, A]] | ||
| + | [[Category: Beta barrel]] | ||
| + | [[Category: Fluorescent protein]] | ||
| + | [[Category: Red fluorescent protein]] | ||
Revision as of 19:37, 9 December 2016
Structure of the Red Fluorescent Protein mScarlet at pH 7.8
| |||||||||||
