1qet
From Proteopedia
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|PDB= 1qet |SIZE=350|CAPTION= <scene name='initialview01'>1qet</scene> | |PDB= 1qet |SIZE=350|CAPTION= <scene name='initialview01'>1qet</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1qes|1QES]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qet OCA], [http://www.ebi.ac.uk/pdbsum/1qet PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qet RCSB]</span> | ||
}} | }} | ||
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[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:12:28 2008'' |
Revision as of 20:12, 30 March 2008
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| Ligands: | , , , | ||||||
| Related: | 1QES
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES
Overview
The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The three-dimensional structures of two representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by two-dimensional NMR and a simulated annealing protocol. Local deviations are similar to other intrahelical GU mismatches with little effect on backbone torsion angles and a slight overtwisting between the base pair 5' of the G of the mismatch and the mismatch itself. Comparisons of the resulting stacking patterns along with electrostatic potential maps suggest that interactions between highly negative electrostatic regions between base pairs may play a role in the observed thermodynamic differences.
About this Structure
1QET is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:9201950
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